Anti-GNAO1 Antibody Picoband®

About this product

Boster Bio Anti-GNAO1 Antibody Picoband® catalog # A05532-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A05532-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Guanine nucleotide-binding protein G (o) subunit alpha is a protein that in humans is encoded by the GNAO1 gene. The protein encoded by this gene represents the alpha subunit of the Go heterotrimeric G-protein signal-transducing complex. Defects in this gene are a cause of early-onset epileptic encephalopathy. Two transcript variants encoding different isoforms have been found for this gene.

Synonyms

Spermine oxidase; Polyamine oxidase 1; PAO-1; PAOh1; Smox; Smo

Gene Name

GNAO1

Gene ID

2775

UniProt

P09471

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human GNAO1 recombinant protein (Position: M1-R179) .

Clonality

Polyclonal

Tissue Specificity

Widely expressed. Isoform 1 and isoform 2 are expressed at higher level in brain and skeletal muscle. Isoform 7 is found in brain and spleen, isoform 10 is widely expressed but found at lower level in heart, kidney, liver and lung.

Applications

WB, ELISA

Field of Research

Chemokines, Immunology, Innate Immunity

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Flavoenzyme which catalyzes the oxidation of spermine to spermidine. Can also use N1-acetylspermine and spermidine as substrates, with different affinity depending on the isoform (isozyme) and on the experimental conditions. Plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs. May contribute to beta-alanine production via aldehyde dehydrogenase conversion of 3-amino-propanal.

References & Citations

1. Ananth, A. L., Robichaux-Viehoever, A., Kim, Y.-M., Hanson-Kahn, A., Cox, R., Enns, G. M., Strober, J., Willing, M., Schlaggar, B. L., Wu, Y. W., Bernstein, J. A. Clinical course of six children with GNAO1 mutations causing a severe and distinctive movement disorder. Pediat. Neurol. 59: 81-84, 2016. 2. Danti, F. R., Galosi, S., Romani, M., Montomoli, M., Carss, K. J., Raymond, F. L., Parrini, E., Bianchini, C., McShane, T., Dale, R. C., Mohammad, S. S., Shah, U., and 9 others. GNAO1 encephalopathy: broadening the phenotype and evaluating treatment and outcome. Neurol. Genet. 3: e143, 2017. Note: Electronic Article. 3. Feng, H., Sjogren, B., Karaj, B., Shaw, V., Gezer, A., Neugib, R. R. Movement disorder in GNAO1 encephalopathy associated with gain-of-function mutations. Neurology 89: 762-770, 2017.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

48608 MW

Observed Molecular Weight

40 kDa

Gene Name Synonym

Spermine oxidase

Subcellular Location

Cytoplasm. Nucleus. Cytoplasm.

Protein Name

Serine protease HTRA3

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.