Anti-KIF4A/4B Antibody Picoband®

About this product

Boster Bio Anti-KIF4A/4B Antibody Picoband® catalog # A04486-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A04486-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Chromosome-associated kinesin KIF4A is a protein that in humans is encoded by the KIF4A gene. This gene encodes a member of the kinesin 4 subfamily of kinesin related proteins. The encoded protein is an ATP dependent microtubule-based motor protein that is involved in the intracellular transport of membranous organelles. This protein also associates with condensed chromosome arms and may be involved in maintaining chromosome integrity during mitosis. This protein may also be involved in the organization of the central spindle prior to cytokinesis. A pseudogene of this gene is found on chromosome X.

Synonyms

Interferon regulatory factor 9; IRF-9; IFN-alpha-responsive transcription factor subunit; ISGF3 p48 subunit; Interferon-stimulated gene factor 3 gamma; ISGF-3 gamma; Transcriptional regulator ISGF3 subunit gamma; IRF9; ISGF3G

Gene Name

KIF4A/4B

Gene ID

24137/285643

UniProt

O95239/Q2VIQ3

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human KIF4A/4B recombinant protein (Position: K729-D1174) .

Clonality

Polyclonal

Tissue Specificity

Leukocytes.

Applications

WB, ICC, IF, ELISA

Field of Research

Cell Adhesion, Cell Type Markers, Cytoskeleton/ECM, Hematopoietic Progenitors, Immunology, Integrins, Myeloid, Signal Transduction, Stem Cells

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Transcription factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta) . Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. IRF9/ISGF3G associates with the phosphorylated STAT1:STAT2 dimer to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.

References & Citations

1. Ha, M. J., Yoon, J., Moon, E., Lee, Y. M., Kim, H. J., Kim, W. Assignment of the kinesin family member 4 genes (KIF4A and KIF4B) to human chromosome bands Xq13.1 and 5q33.1 by in situ hybridization. Cytogenet. Cell Genet. 88: 41-42, 2000. 2. Kim, W., Tang, Y., Okada, Y., Torrey, T. A., Chattopadhyay, S. K., Pfleiderer, M., Falkner, F. G., Dorner, F., Choi, W., Hirokawa, N., Morse, H. C., III. Binding of murine leukemia virus Gag polyproteins to KIF4, a microtubule-based motor protein. J. Virol. 72: 6898-6901, 1998. 3. Kurasawa, Y., Earnshaw, W. C., Mochizuki, Y., Dohmae, N., Todokoro, K. Essential roles of KIF4 and its binding partner PRC1 in organized central spindle midzone formation. EMBO J. 23: 3237-3248, 2004.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

43696 MW

Observed Molecular Weight

140 kDa

Gene Name Synonym

Interferon regulatory factor 9

Subcellular Location

Cytoplasm . Nucleus . Translocated into the nucleus upon activation by IFN-alpha/beta.

Protein Name

Interferon regulatory factor 9

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.