Anti-KLF15 Antibody Picoband®

About this product

Boster Bio Anti-KLF15 Antibody Picoband® catalog # A03661-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A03661-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Kruppel-like factor 15 is a protein that in humans is encoded by the KLF15 gene in the Kruppel-like factor family. KLF15, a kidney-enriched Krüppel-like zinc finger-containing transcription factors, is a transcriptonal activator that binds the CLCNKA promoter. The nuclear protein KLF15, is expressed primarily in liver, heart, skeletal muscle and kidney tissues but is not detected in lymphoid tissues or bone marrow. It is an important regulator of GLUT4 in both adipose and muscle tissues.Although the calculated molecular mass of KLF15 is 44 kDa, the apparent molecular mass on SDS–PAGE is 65 kDa due to the post-translational modifications.

Synonyms

DNA replication licensing factor MCM5; CDC46 homolog; P1-CDC46; MCM5; CDC46

Gene Name

KLF15

Gene ID

28999

UniProt

Q9UIH9

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human KLF15 recombinant protein (Position: M1-C323) .

Clonality

Polyclonal

Tissue Specificity

Ubiquitous.

Applications

WB, IHC, ELISA

Field of Research

ER Proteins, Golgi Proteins, Protein Trafficking, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Interacts with MCMBP.

References & Citations

1. Dmitriev, P., Petrov, A., Ansseau, E., Stankevicins, L., Charron, S., Kim, E., Bos, T. J., Robert, T., Turki, A., Coppee, F., Belayew, A., Lazar, V., Carnac, G., Laoudj, D., Lipinski, M., Vassetzky, Y. S. The Kruppel-like factor 15 as a molecular link between myogenic factors and a chromosome 4q transcriptional enhancer implicated in facioscapulohumeral dystrophy. J. Biol. Chem. 286: 44620-44631, 2011. 2. Fisch, S., Gray, S., Heymans, S., Haldar, S. M., Wang, B., Pfister, O., Cui, L., Kumar, A., Lin, Z., Sen-Banerjee, S., Das, H., Petersen, C. A., Mende, U., Burleigh, B. A., Zhu, Y., Pinto, Y. M., Liao, R., Jain, M. K. Kruppel-like factor 15 is a regulator of cardiomyocyte hypertrophy. Proc. Nat. Acad. Sci. 104: 7074-7079, 2007. Note: Erratum: Proc. Nat. Acad. Sci. 104: 13851 only, 2007. 3. Jeyaraj, D., Haldar, S. M., Wan, X., McCauley, M. D., Ripperger, J. A., Hu, K., Lu, Y., Eapen, B. L., Sharma, N., Ficker, E., Cutler, M. J., Gulick, J., and 9 others. Circadian rhythms govern cardiac repolarization and arrhythmogenesis. Nature 483: 96-99, 2012.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

28461 MW

Observed Molecular Weight

44 kDa

Gene Name Synonym

Minichromosome maintenance complex component 5

Subcellular Location

Nucleus. Cytosol.

Protein Name

Breast cancer metastasis-suppressor 1

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.