Anti-KLHL12 Antibody Picoband®

About this product

Boster Bio Anti-KLHL12 Antibody Picoband® catalog # A08568-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A08568-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Kelch-like protein 12 is a protein that in humans is encoded by the KLHL12 gene. This gene encodes a member of the KLHL (Kelch-like) family of proteins. This protein has been identified as an autoantigen in the autoimmune disease Sjogren's syndrome and as a potential biomarker in primary biliary cirrhosis. This protein may act as a substrate adaptor of the Cullin-3 ubiquitin ligase complex to promote substrate-specific ubiquitylation. Ubiquitylation by this complex has been shown to regulate the Wnt signaling pathway as well as COPII vesicle coat size. A pseudogene has been identified on chromosome 22. Alternative splicing results in multiple transcript variants.

Synonyms

Gap junction gamma-1 protein; Connexin-45; Cx45; Gap junction alpha-7 protein; GJC1; GJA7

Gene Name

KLHL12

Gene ID

59349

UniProt

Q53G59

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human KLHL12 recombinant protein (Position: R27-I331) .

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed.

Applications

ELISA, Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Cell Adhesion, Cytoskeleton/ECM, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

References & Citations

1. Hartz, P. A. Personal Communication. Baltimore, Md. 3/7/2012. 2. Jin, L., Pahuja, K. B., Wickliffe, K. E., Gorur, A., Baumgartel, C., Schekman, R., Rape, M. Ubiquitin-dependent regulation of COPII coat size and function. Nature 482: 495-500, 2012. 3. Mai, A., Jung, S.-K., Yonehara, S. hDKIR, a human homologue of the Drosophila kelch protein, involved in a ring-like structure. Exp. Cell Res. 300: 72-83, 2004.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

45470 MW

Observed Molecular Weight

63 kDa

Applications Notes

6

Gene Name Synonym

Gap junction gamma-1 protein

Subcellular Location

Cell membrane; Multi-pass membrane protein. Cell junction, gap junction.

Protein Name

Gap junction gamma-1 protein

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.