Anti-PAK3 Antibody Picoband®

About this product

Boster Bio Anti-PAK3 Antibody Picoband® catalog # A03124-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A03124-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Serine/threonine-protein kinase PAK 3is anenzymethat in humans is encoded by thePAK3gene. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities. The protein encoded by this gene forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1 proteins which then catalyzes a variety of targets. This protein may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Defects in this gene are the cause of non-syndromic mental retardation X-linked type 30 (MRX30), also called X-linked mental retardation type 47 (MRX47) . Alternatively spliced transcript variants encoding different isoforms have been identified.

Synonyms

Eosinophil cationic protein; ECP; Ribonuclease 3; RNase 3; RNASE3; ECP; RNS3

Gene Name

PAK3

Gene ID

5063

UniProt

O75914

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human PAK3 recombinant protein (Position: M1-D263) .

Clonality

Polyclonal

Tissue Specificity

Mainly expressed in pachytene spermatocytes of testis and in lymphocyte-rich areas of spleen and lymph nodes. Isoform v1 is expressed in spleen. Isoform v2 is expressed in testis. Also detected in ovary, placenta, pancreas, cardiac fibroblasts. Expressed in B-cells and prostate malignant cells. Isoform v1 and isoform v3 are expressed in epithelial colorectal adenocarcinoma cells. Isoform v2 and isoform v4 are expressed in endothelial cells. Isoform v1, isoform v2, isoform v3 and isoform v4 are expressed in pulmonary artery smooth muscle cells. Isoform v2 and isoform v5 are expressed in microvascular endothelial cells (at protein level) .

Applications

WB, IHC, ICC, IF, Flow Cytometry, ELISA

Field of Research

2339, DNA/RNA, Epigenetics and Nuclear Signaling, Nuclear Receptors, Nuclear Signaling Pathways, RNA Processing

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.

References & Citations

1. Entrez Gene: PAK3 p21 (CDKN1A) -activated kinase 3. 2. Allen KM, Gleeson JG, Bagrodia S, Partington MW, MacMillan JC, Cerione RA, Mulley JC, Walsh CA (Oct 1998) . PAK3 mutation in nonsyndromic X-linked mental retardation. Nat Genet 20 (1) : 25–30. 3. Donnelly AJ, Partington MW, Ryan AK, Mulley JC (Nov 1996) . Regional localisation of two non-specific X-linked mental retardation genes (MRX30 and MRX31) . Am J Med Genet 64 (1) : 113–20.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

64406 MW

Observed Molecular Weight

62 kDa

Applications Notes

6

Gene Name Synonym

Ribonuclease A family member 3

Subcellular Location

Secreted. Located in the matrix of eosinophil large specific granule, which are released following activation by an immune stimulus.

Protein Name

Transcription factor E2F3

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.