Anti-PKA R2/PKR2/PRKAR2A Antibody Picoband®

About this product

Boster Bio Anti-PKA R2/PKR2/PRKAR2A Antibody Picoband® catalog # A03911-2. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A03911-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CAMP-dependent protein kinase type II-alpha regulatory subunit is an enzyme that in humans is encoded by the PRKAR2A gene. cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER) .

Synonyms

Fructose-bisphosphate aldolase B; Liver-type aldolase; ALDOB; ALDB

Gene Name

PRKAR2A

Gene ID

5576

UniProt

P13861

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human PKA R2/PKR2/PRKAR2A recombinant protein (Position: E20-K315) .

Clonality

Polyclonal

Tissue Specificity

Pancreas-specific (at protein level) . Loss of expression is seen in ductal type pancreas cancers.

Applications

ELISA, Flow Cytometry, IF, ICC, WB

Field of Research

Cancer, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Transcription factor implicated in the cell fate determination in various organs. Binds to the E-box consensus sequence 5'-CANNTG-3'. Plays a role in early and late pancreas development and differentiation. Important for determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. May be involved in the maintenance of exocrine pancreas-specific gene expression including ELA1 and amylase. Required for the formation of pancreatic acinar and ductal cells. Plays an important role in cerebellar development. ly regulated by FOXN4 and RORC during retinal development, FOXN4-PTF1A pathway plays a central role in ing the differentiation of retinal progenitors towards horizontal and amacrine fates.

References & Citations

1. Bossis, I., Stratakis, C. A. PRKAR1A: normal and abnormal functions. Endocrinology 145: 5452-5458, 2004. 2. Oyen, O., Myklebust, F., Scott, J. D., Hansson, V., Jahnsen, T. Human testis cDNA for the regulatory subunit RII alpha of cAMP-dependent protein kinase encodes an alternate amino-terminal region. FEBS Lett. 246: 57-64, 1989. 3. Tasken, K., Naylor, S. L., Solberg, R., Jahnsen, T. Mapping of the gene encoding the regulatory subunit RII-alpha of cAMP-dependent protein kinase (locus PRKAR2A) to human chromosome region 3p21.3-p21.2. Genomics 50: 378-381, 1998.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

52 kDa

Applications Notes

6

Gene Name Synonym

Aldolase, fructose-bisphosphate B

Subcellular Location

Nucleus. Cytoplasm.

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.