Anti-AKAP 95/AKAP8 Antibody Picoband®

About this product

Boster Bio Anti-AKAP 95/AKAP8 Antibody Picoband® catalog # A05133-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A05133-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

A-kinase anchor protein 8 is an enzyme that, in humans, is encoded by the AKAP8 gene. This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. This gene encodes a nuclear A-kinase anchor protein that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of this gene is located on the short arm of chromosome 9.

Synonyms

Sodium- and chloride-dependent GABA transporter 1; GAT-1; Solute carrier family 6 member 1; SLC6A1; GABATR, GABT1, GAT1

Gene Name

AKAP8

Gene ID

10270

UniProt

O43823

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human AKAP 95/AKAP8 recombinant protein (Position: T380-F546) .

Clonality

Polyclonal

Tissue Specificity

Expressed in many tissues, highest levels in skeletal muscle.

Applications

WB, IHC, ICC, IF, Flow Cytometry, ELISA

Field of Research

Neuroscience, Neurotransmitter, Transporters

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.

References & Citations

1. Eide, T., Coghlan, V., Orstavik, S., Holsve, C., Solberg, R., Skalhegg, B. S., Lamb, N. J. C., Langeberg, L., Fernandez, A., Scott, J. D., Jahnsen, T., Tasken, K. Molecular cloning, chromosomal localization, and cell cycle-dependent subcellular distribution of the A-kinase anchoring protein, AKAP95. Exp. Cell Res. 238: 305-316, 1998.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

67074 MW

Observed Molecular Weight

92 kDa

Applications Notes

6

Gene Name Synonym

Sodium- and chloride-dependent GABA transporter 1

Subcellular Location

Cell membrane; Multi-pass membrane protein. Membrane; Multi-pass membrane protein. Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons (By similarity) . .

Protein Name

Long-chain-fatty-acid--CoA ligase 5

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.