Anti-Fibrillarin/FBL Antibody Picoband®

About this product

Boster Bio Anti-Fibrillarin/FBL Antibody Picoband® catalog # A03178-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A03178-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

RRNA 2'-O-methyltransferase fibrillarin is an enzyme that in humans is encoded by the FBL gene. This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin.

Synonyms

IFNGR2 protein; Interferon gamma receptor 2; IFNGR2; mCG_11456

Gene Name

FBL

Gene ID

2091

UniProt

P22087

Host

Rabbit

Reactivity

Human, Monkey

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Fibrillarin/FBL recombinant protein (Position: K77-N138) .

Clonality

Polyclonal

Tissue Specificity

Wide tissue distribution (highest in the pancreas and very low in brain) . Closely associated with the basal layer of cells in epithelia and the endothelium of blood vessel walls.

Applications

WB, ICC, IF, Flow Cytometry, ELISA

Field of Research

Adaptive Immunity, Immunology, T Cells

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1. Requires primed phosphorylation of the majority of its substrates. Contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Regulates glycogen metabolism in liver, but not in muscle. May also mediate the development of insulin resistance by regulating activation of transcription factors. In Wnt signaling, regulates the level and transcriptional activity of nuclear CTNNB1/beta-catenin. Facilitates amyloid precursor protein (APP) processing and the generation of APP-derived amyloid plaques found in Alzheimer disease. May be involved in the regulation of replication in pancreatic beta-cells. Is necessary for the establishment of neuronal polarity and axon outgrowth. Through phosphorylation of the anti-apoptotic protein MCL1, may control cell apoptosis in response to growth factors deprivation. Acts as a regulator of autophagy by mediating phosphorylation of KAT5/TIP60 under starvation conditions, leading to activate KAT5/TIP60 acetyltransferase activity and promote acetylation of key autophagy regulators, such as ULK1 and RUBCNL/Pacer.

References & Citations

1. Aris, J. P., Blobel, G. cDNA cloning and sequencing of human fibrillarin, a conserved nucleolar protein recognized by autoimmune antisera. Proc. Nat. Acad. Sci. 88: 931-935, 1991. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 7/9/2010. 3. Okano, Y., Steen, V. D., Medsger, T. A., Jr. Autoantibody to U3 nucleolar ribonucleoprotein (fibrillarin) in patients with systemic sclerosis. Arthritis Rheum. 35: 95-100, 1992.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

65411 MW

Observed Molecular Weight

37 kDa

Applications Notes

6

Gene Name Synonym

Interferon gamma receptor 2

Subcellular Location

Plasma membrane. Integral component of membrane.

Protein Name

Glutamate decarboxylase 2

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.