Anti-RAE1 Antibody Picoband®

About this product

Boster Bio Anti-RAE1 Antibody Picoband® catalog # A04228-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A04228-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

MRNA export factor is a protein that in humans is encoded by the RAE1 gene. Mutations in the Schizosaccharomyces pombe Rae1 and Saccharomyces cerevisiae Gle2 genes have been shown to result in accumulation of poly (A) -containing mRNA in the nucleus, suggesting that the encoded proteins are involved in RNA export. The protein encoded by this gene is a homolog of yeast Rae1. It contains four WD40 motifs, and has been shown to localize to distinct foci in the nucleoplasm, to the nuclear rim, and to meshwork-like structures throughout the cytoplasm. This gene is thought to be involved in nucleocytoplasmic transport, and in ly or inly attaching cytoplasmic mRNPs to the cytoskeleton. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

Synonyms

Mucosal addressin cell adhesion molecule 1; MAdCAM-1; hMAdCAM-1; MADCAM1

Gene Name

RAE1

Gene ID

8480

UniProt

P78406

Host

Rabbit

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human RAE1 recombinant protein (Position: Q76-K368) .

Clonality

Polyclonal

Tissue Specificity

Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes.

Applications

WB, IHC, ICC, IF, Flow Cytometry, ELISA

Field of Research

Angiogenesis, Cardiovascular, Cell Adhesion, Cell Adhesion Proteins, Cytoskeleton/ECM, Immunology, Innate Immunity, Integrins, Macrophage/Inflammation, Neuroscience, Receptors, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Cell adhesion leukocyte receptor expressed by mucosal venules, helps to lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L- selectin binding.

References & Citations

1. Bharathi, A., Ghosh, A., Whalen, W. A., Yoon, J. H., Pu, R., Dasso, M., Dhar, R. The human RAE1 gene is a functional homologue of Schizosaccharomyces pombe rae1 gene involved in nuclear export of poly (A) + RNA. Gene 198: 251-258, 1997. 2. Jeganathan, K. B., Malureanu, L., van Deursen, J. M. The Rae1-Nup98 complex prevents aneuploidy by inhibiting securin degradation. Nature 438: 1036-1039, 2005. 3. Kraemer, D., Blobel, G. mRNA binding protein mrnp 41 localizes to both nucleus and cytoplasm. Proc. Nat. Acad. Sci. 94: 9119-9124, 1997.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

41 kDa

Applications Notes

6

Gene Name Synonym

Mucosal vascular addressin cell adhesion molecule 1

Subcellular Location

Membrane; Single-pass type I membrane protein.

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.