Anti-RPL29 Antibody Picoband®

About this product

Boster Bio Anti-RPL29 Antibody Picoband® catalog # A05949-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A05949-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

60S ribosomal protein L29 is a protein that in humans is encoded by the RPL29 gene. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 60S subunit. The protein belongs to the L29E family of ribosomal proteins. The protein is also a peripheral membrane protein expressed on the cell surface that ly binds heparin. Although this gene was previously reported to map to 3q29-qter, it is believed that it is located at 3p21.3-p21.2. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Synonyms

Long-chain-fatty-acid--CoA ligase 3;6.2.1.3; Long-chain acyl-CoA synthetase 3; LACS 3; ACSL3; ACS3, FACL3, LACS3

Gene Name

RPL29

Gene ID

6159

UniProt

P47914

Host

Rabbit

Reactivity

Human, Mouse, Rat, Monkey

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human RPL29 recombinant protein (Position: Q27-A153) .

Clonality

Polyclonal

Tissue Specificity

Expressed in breast, ductal and invasive ductal carcinomas of the breast, sporadic colorectal adenomas and carcinomas (at protein level) . Expressed in fetal brain. Expressed in lung, amygdala, eye, prostate, pancreatic and prostate cancers, head and neck tumors and embryonal tumor.

Applications

WB, IHC, ICC, IF, Flow Cytometry, ELISA

Field of Research

Cardiovascular, Fatty Acid Oxidation, Fatty Acids, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Redox Metabolism, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Acyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis (By similarity) . Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates (By similarity) . Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins) . .

References & Citations

1. Garcia-Barcelo, M., Law, P. T. W., Tsui, S. K. W., Fung, K. P., Lee, C. Y., Waye, M. M. Y. Mapping of the human ribosomal large subunit protein gene RPL29 to human chromosome 3q29-qter. Genomics 46: 148-151, 1997. 2. Kirn-Safran, C. B., Dayal, S., Martin-DeLeon, P. A., Carson, D. D. Cloning, expression, and chromosome mapping of the murine Hip/Rpl29 gene. Genomics 68: 210-219, 2000. 3. Law, P. T. W., Tsui, S. K. W., Lam, W. Y., Luk, S. C. W., Hwaang, D. M., Liew, C. C., Lee, C. Y., Fung, K. P., Waye, M. M. Y. A novel cDNA encoding a human homologue of ribosomal protein L29. Biochim. Biophys. Acta 1305: 105-108, 1996.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

80420 MW

Observed Molecular Weight

20 kDa

Applications Notes

6

Gene Name Synonym

Long-chain-fatty-acid--CoA ligase 3

Subcellular Location

Mitochondrion outer membrane ; Single-pass type III membrane protein . Peroxisome membrane ; Single-pass type III membrane protein . Microsome membrane ; Single-pass type III membrane protein . Endoplasmic reticulum membrane ; Single-pass type III membrane protein .

Protein Name

Long-chain-fatty-acid--CoA ligase 3

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.