Anti-hUPF2/RENT2/UPF2 Antibody Picoband®

About this product

Boster Bio Anti-hUPF2/RENT2/UPF2 Antibody Picoband® catalog # A03627-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A03627-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Regulator of nonsense transcripts 2 is a protein that in humans is encoded by the UPF2 gene. This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD) . When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located in the perinuclear area. It interacts with translation release factors and the proteins that are functional homologs of yeast Upf1p and Upf3p. Two splice variants have been found for this gene; both variants encode the same protein.

Synonyms

Protocadherin-15; PCDH15; USH1F

Gene Name

UPF2

Gene ID

26019

UniProt

Q9HAU5

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human hUPF2/RENT2/UPF2 recombinant protein (Position: K200-H651) .

Clonality

Polyclonal

Tissue Specificity

Expressed in brain, lung, kidney, spleen and testis. Found also in the inner and outer synaptic layers, and the nerve fiber layer in adult and fetal retinas. Found in the supporting cells, outer sulcus cells and spiral ganglion of fetal cochlea. Expressed in cytotoxic tumor-derived T- and NK-cell lines as well as biopsies of nasal NK/T-cell lymphomas. Not detected in normal or in vitro activated peripheral blood cells, CD4 or CD8 lymphocytes or NK cells. Isoform 3 is expressed in brain, heart, cerebellum and kidney. CD1 isoforms, such as isoform 1, have a limited pattern of expression and is detected in testis, retina and cochlea. CD2 isoforms, such as isoforms 4 and 5, are expressed in heart, kidney, thymus, spleen, testis, retina and cochlea. CD3 isoforms, such as isoform 6, are widely expressed.

Applications

ELISA, Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Cancer Susceptibility, Epigenetics and Nuclear Signaling, Transcription, Tumor Suppressors

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Calcium-dependent cell-adhesion protein. Essential for maintenance of normal retinal and cochlear function.

References & Citations

1. Johnson, J. L., Stoica, L., Liu, Y., Zhu, P. J., Bhattacharya, A., Buffington, S. A., Huq, R., Eissa, N. T., Larsson, O., Porse, B. T., Domingo, D., Nawaz, U., and 19 others. Inhibition of Upf2-dependent nonsense-mediated decay leads to behavioral and neurophysiological abnormalities by activating the immune response. Neuron 104: 665-679, 2019. 2. Lykke-Andersen, J., Shu, M.-D., Steitz, J. A. Human Upf proteins target an mRNA for nonsense-mediated decay when bound downstream of a termination codon. Cell 103: 1121-1131, 2000. 3. Mendell, J. T., ap Rhys, C. M. J., Dietz, H. C. Separable roles for rent1/hUpf1 in altered splicing and decay of nonsense transcripts. Science 298: 419-371, 2002.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

28461 MW

Observed Molecular Weight

170 kDa

Applications Notes

6

Gene Name Synonym

Protocadherin related 15

Subcellular Location

Cell membrane. Single-pass type I. membrane protein. Secreted.

Protein Name

Breast cancer metastasis-suppressor 1

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.