Anti-Cytokeratin 5 Antibody Picoband® (monoclonal, 5D3F7)

About this product

Boster Bio Anti-Cytokeratin 5 Antibody Picoband® (monoclonal, 5D3F7) catalog # M00398-6. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M00398-6

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cytokeratin 5, also known as KRT5, K5, or CK5, is a protein that is encoded in humans by the KRT5 gene. The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Synonyms

Interferon gamma; IFN-gamma; IFNG

Gene Name

KRT5

Gene ID

3852

UniProt

P13647

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Cytokeratin 5, different from the related mouse sequence by one amino acid, and identical to the related rat sequence.

Clonality

Monoclonal

Clone

Clone: 5D3F7

Tissue Specificity

Released primarily from activated T lymphocytes.

Applications

WB, IHC, ICC, IF, Flow Cytometry

Field of Research

Cancer, Cytokines, Immunology, Innate Immunity, Interferons, Signaling Pathways, Stem Cells, TGF Beta, Tumor Immunology

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

References & Citations

1. Entrez Gene: KRT5 keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types) . 2. Eckert RL, Rorke EA (Jun 1988) . The sequence of the human epidermal 58-kD (#5) type II keratin reveals an absence of 5' upstream sequence conservation between coexpressed epidermal keratins. Dna. 7 (5) : 337–45. 3. Lersch R, Fuchs E (Jan 1988) . Sequence and expression of a type II keratin, K5, in human epidermal cells. Molecular and Cellular Biology. 8 (1) : 486–93.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

62 kDa

Applications Notes

6

Gene Name Synonym

Interferon, gamma

Subcellular Location

Secreted.

Protein Name

Membrane cofactor protein

Isotype

Mouse IgG2b

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.