Anti-PCNA Antibody Picoband® (monoclonal, 2G2)

About this product

Boster Bio Anti-PCNA Antibody Picoband® (monoclonal, 2G2) catalog # M00125-3. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M00125-3

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. It is mapped to 20p12.3. The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Synonyms

Interleukin-6; IL-6; B-cell stimulatory factor 2; BSF-2; CTL differentiation factor; CDF; Hybridoma growth factor; Interferon beta-2; IFN-beta-2; IL6; IFNB2

Gene Name

PCNA

Gene ID

5111

UniProt

P12004

Host

Mouse

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human PCNA recombinant protein (Position: M1-S261) .

Clonality

Monoclonal

Clone

Clone: 2G2

Tissue Specificity

Ubiquituous. Expressed in platelets.

Applications

WB, IHC, ICC, IF, Flow Cytometry

Field of Research

Cadherins, Calcium Binding Proteins, Calcium Signaling, Cancer, Cell Adhesion, Cytoskeleton/ECM, Invasion/Microenvironment, Signal Transduction, Signaling Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T (H) 17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation.

References & Citations

1. Moldovan GL, Pfander B, Jentsch S (May 18, 2007) . PCNA, the maestro of the replication fork. Cell. 129 (4) : 665–79. 2. Warbrick E (Mar 1998) . PCNA binding through a conserved motif. BioEssays. 20 (3) : 195–9. 3. Mailand N, Gibbs-Seymour I, Bekker-Jensen S (May 2013) . Regulation of PCNA-protein interactions for genome stability. Nature Reviews. Molecular Cell Biology. 14 (5) : 269–82.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

36 kDa

Applications Notes

6

Gene Name Synonym

Interleukin 6

Subcellular Location

Secreted

Isotype

Mouse IgG2b

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.