Anti-ERCC1 Antibody Picoband®

About this product

Boster Bio Anti-ERCC1 Antibody Picoband® catalog # A00388-3. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00388-3

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

DNA excision repair protein ERCC-1 is a protein that in humans is encoded by the ERCC1 gene. The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand.

Synonyms

DNA excision repair protein ERCC-1; ERCC1

Gene Name

ERCC1

Gene ID

2067

UniProt

P07992

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human ERCC1 recombinant protein (Position: R106-E291) .

Clonality

Polyclonal

Tissue Specificity

Expressed in fetal brain.

Applications

WB, ICC, IF, Flow Cytometry, ELISA

Field of Research

Cancer, DNA/RNA, DNA Damage & Repair, Epigenetics and Nuclear Signaling, Tumor Biomarkers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Isoform 1: Non-catalytic component of a structure- specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL) . Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-ed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4.

References & Citations

1. Imoto, K., Boyle, J., Oh, K., Khan, S., Ueda, T., Nadem, C., Slor, H., Orgal, S., Gadoth, N., Busch, D., Jaspers, N. G., Tamura, D., DiGiovanna, J. J., Kraemer, K. H. Patients with defects in the interacting nucleotide excision repair proteins ERCC1 or XPF show xeroderma pigmentosum with late onset severe neurological degeneration. (Abstract) J. Invest. Derm. 127: S92 only, 2007. 2. McCabe, K. M., Hemphill, A., Akkari, Y., Jakobs, P. M., Pauw, D., Olson, S. B., Moses, R. E., Grompe, M. ERCC1 is required for FANCD2 focus formation. Molec. Genet. Metab. 95: 66-73, 2008.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

17628 MW

Observed Molecular Weight

33 kDa

Applications Notes

6

Gene Name Synonym

ERCC excision repair 1, endonuclease non-catalytic subunit

Subcellular Location

Isoform 1: Nucleus.

Protein Name

Interleukin-2

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.