Anti-CLLD8/Setdb2 Antibody Picoband®

About this product

Boster Bio Anti-CLLD8/Setdb2 Antibody Picoband® catalog # A08290-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A08290-3

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

SET domain bifurcated histone lysine methyltransferase 2 is a protein in humans encoded by the SETDB2 gene. It is mapped to 13q14.2. This gene encodes a member of a family of proteins that contain a methyl-CpG-binding domain (MBD) and a SET domain and function as histone methyltransferases. This protein is recruited to heterochromatin and plays a role in the regulation of chromosome segregation. This region is commonly deleted in chronic lymphocytic leukemia. Naturally-occuring readthrough transcription occurs from this gene to the downstream PHF11 (PHD finger protein 11) gene. Alternative splicing results in multiple transcript variants.

Synonyms

Histone-lysine N-methyltransferase SETDB2; Chronic lymphocytic leukemia deletion region gene 8 protein; Lysine N-methyltransferase 1F; SET domain bifurcated 2; SETDB2; C13orf4; CLLD8; KMT1F

Gene Name

Setdb2

Gene ID

100361710

UniProt

M0RA29

Host

Rabbit

Reactivity

Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived rat CLLD8/Setdb2 recombinant protein (Position: K20-I714) .

Clonality

Polyclonal

Tissue Specificity

Highly expressed in reproductive organs, such as testis, ovary and prostate.

Applications

ELISA, Flow Cytometry, WB

Field of Research

Immune System Diseases, Immunology, Protein Trafficking, Signal Transduction, Signaling Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Histone methyltransferase involved in left-right axis specification in early development and mitosis. Specifically trimethylates 'Lys-9' of histone H3 (H3K9me3) . H3K9me3 is a specific tag for epigenetic transcriptional repression that recruits HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Contributes to H3K9me3 in both the interspersed repetitive elements and centromere-associated repeats. Plays a role in chromosome condensation and segregation during mitosis.

References & Citations

1. Mabuchi, H., Fujii, H., Calin, G., Alder, H., Negrini, M., Rassenti, L., Kipps, T. J., Bullrich, F., Croce, C. M. Cloning and characterization of CLLD6, CLLD7, CLLD8, novel candidate genes for leukemogenesis at chromosome 13q14, a region commonly deleted in B-cell chronic lymphocytic leukemia. Cancer Res. 61: 2870-2877, 2001. 2. Zhang, Y., Leaves, N. I., Anderson, G. G., Ponting, C. P., Broxholme, J., Holt, R., Edser, P., Bhattacharyya, S., Dunham, A., Adcock, I. M., Pulleyn, L., Barnes, P. J., and 11 others. Positional cloning of a quantitative trait locus on chromosome 13q14 that influences immunoglobulin E levels and asthma. Nature Genet. 34: 181-186, 2003.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

100 kDa

Applications Notes

6

Gene Name Synonym

SET domain bifurcated histone lysine methyltransferase 2

Subcellular Location

Nucleus. Chromosome.

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.