Anti-SGT1/ECD Antibody Picoband®

About this product

Boster Bio Anti-SGT1/ECD Antibody Picoband® catalog # A00567-2. Tested in ELISA, Flow Cytometry, IP, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00567-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Protein SGT1 is a protein that in humans is encoded by the ECD gene. Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP. May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes. Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins. The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex. May play a role in regulation of pre-mRNA splicing.

Synonyms

Disintegrin and metalloproteinase domain-containing protein 10; ADAM 10; CDw156; Kuzbanian protein homolog; Mammalian disintegrin-metalloprotease; CD156c; ADAM10; KUZ; MADM

Gene Name

ECD

Gene ID

11319

UniProt

O95905

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human SGT1/ECD recombinant protein (Position: L50-Q567) .

Clonality

Polyclonal

Tissue Specificity

Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver.

Applications

WB, ICC, IF, IP, Flow Cytometry, ELISA

Field of Research

Apoptosis, Cancer, Cell Biology, Cell Death, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Metalloprotease, Proteolysis/Ubiquitin, Proteolytic Enzymes, Receptors, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface (PubMed:20592283) . Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP) (PubMed:26686862, PubMed:11786905) . Contributes to the normal cleavage of the cellular prion protein (PubMed:11477090) . Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity (PubMed:12475894) . Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis (By similarity) . Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form (PubMed:17557115) . Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B (PubMed:19114711, PubMed:21288900) . May regulate the EFNA5-EPHA3 signaling (PubMed:16239146) .

References & Citations

1. Hartz, P. A. Personal Communication. Baltimore, Md. 7/9/2015. 2. Kim, J. H., Gurumurthy, C. B., Band, H., Band, V. Biochemical characterization of human Ecdysoneless reveals a role in transcriptional regulation. Biol. Chem. 391: 9-19, 2010. 3. Sato, T., Jigami, Y., Suzuki, T., Uemura, H. A human gene, hSGT1, can substitute for GCR2, which encodes a general regulatory factor of glycolytic gene expression in Saccharomyces cerevisiae. Molec. Gen. Genet. 260: 535-540, 1999.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

73 kDa

Observed Molecular Weight

73 kDa

Applications Notes

6

Gene Name Synonym

ADAM metallopeptidase domain 10

Subcellular Location

Cell membrane.

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.