Anti-MUS81 Antibody Picoband®

About this product

Boster Bio Anti-MUS81 Antibody Picoband® catalog # A00810-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00810-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Crossover junction endonuclease MUS81 is an enzyme that in humans is encoded by the MUS81 gene. This gene encodes a structure-specific endonuclease which belongs to the XPF/MUS81 endonuclease family and plays a critical role in the resolution of recombination intermediates during DNA repair after inter-strand cross-links, replication fork collapse, and DNA double-strand breaks. The encoded protein associates with one of two closely related essential meiotic endonuclease proteins (EME1 or EME2) to form a complex that processes DNA secondary structures. It contains an N-terminal DEAH helicase domain, an excision repair cross complementation group 4 (ERCC4) endonuclease domain, and two tandem C-terminal helix-hairpin-helix domains. Mice with a homozygous knockout of the orthologous gene have significant meiotic defects including the failure to repair a subset of DNA double strand breaks.

Synonyms

Lipopolysaccharide-binding protein; LBP; Lbp

Gene Name

MUS81

Gene ID

80198

UniProt

Q96NY9

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human MUS81 recombinant protein (Position: H283-R483) .

Clonality

Polyclonal

Tissue Specificity

Detected in blood serum (at protein level) .

Applications

WB, ELISA

Field of Research

Defensin, Human Protein, Immunology, Innate Immunity, Macrophage/Inflammation, Microbiology, Protein, TLR Signaling

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria (PubMed:9144073) . Acts as an affinity enhancer for CD14, facilitating its association with LPS (By similarity) . Promotes the release of cytokines in response to bacterial lipopolysaccharide (PubMed:24380872) .

References & Citations

1. Chen, X.-B., Melchionna, R., Denis, C.-M., Gaillard, P.-H. L., Blasina, A., Van de Weyer, I., Boddy, M. N., Russell, P., Vialard, J., McGowan, C. H. Human Mus81-associated endonuclease cleaves Holliday junctions in vitro. Molec. Cell 8: 1117-1127, 2001. 2. Ciccia, A., Constantinou, A., West, S. C. Identification and characterization of the human Mus81-Eme1 endonuclease. J. Biol. Chem. 278: 25172-25178, 2003. 3. Ciccia, A., Ling, C., Coulthard, R., Yan, Z., Xue, Y., Meetei, A. R., Laghmani, E. H., Joenje, H., McDonald, N., de Winter, J. P., Wang, W., West, S. C. Identification of FAAP24, a Fanconi anemia core complex protein that interacts with FANCM. Molec. Cell 25: 331-343, 2007.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

53384 MW

Observed Molecular Weight

52-72 kDa

Gene Name Synonym

Lipopolysaccharide binding protein

Subcellular Location

Secreted

Protein Name

Lipopolysaccharide-binding protein

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.