Anti-PDGF AA/PDGFA Antibody Picoband®

About this product

Boster Bio Anti-PDGF AA/PDGFA Antibody Picoband® catalog # A02257-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02257-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Platelet-derived growth factor subunit A is a protein that in humans is encoded by the PDGFA gene. This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF) . The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit A, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit B. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Alternative splicing results in multiple transcript variants.

Synonyms

Mitochondrial uncoupling protein 2; UCP 2; Solute carrier family 25 member 8; UCPH; UCP2; SLC25A8

Gene Name

PDGFA

Gene ID

5154

UniProt

P04085

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human PDGF AA/PDGFA recombinant protein (Position: S87-T211) .

Clonality

Polyclonal

Tissue Specificity

Widely expressed in adult human tissues, including tissues rich in macrophages. Most expressed in white adipose tissue and skeletal muscle.

Applications

WB, ICC, IF, ELISA

Field of Research

Cancer, Cancer Metabolism, Energy Transfer Pathways, Integration Of Energy, Integration Of Energy Metabolism, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Obesity, Pathways and Processes

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat.

References & Citations

1. Beckmann, M. P., Betsholtz, C., Heldin, C.-H., Westermark, B., Di Marco, E., Di Fiore, P. P., Robbins, K. C., Aaronson, S. A. Comparison of biological properties and transforming potential of human PDGF-A and PDGF-B chains. Science 241: 1346-1349, 1988. 2. Betsholtz, C., Johnsson, A., Heldin, C.-H., Westermark, B., Lind, P., Urdea, M. S., Eddy, R., Shows, T. B., Philpott, K., Mellor, A. L., Knott, T. J., Scott, J. cDNA sequence and chromosomal localization of human platelet-derived growth factor A-chain and its expression in tumour cell lines. Nature 320: 695-699, 1986. 3. Bonthron, D., Collins, T., Grzeschik, K.-H., van Roy, N., Speleman, F. Platelet-derived growth factor A chain: confirmation of localization of PDGFA to chromosome 7p22 and description of an unusual minisatellite. Genomics 13: 257-263, 1992.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

33229 MW

Observed Molecular Weight

24 kDa

Gene Name Synonym

Mitochondrial uncoupling protein 2

Subcellular Location

Mitochondrion inner membrane; Multi-pass membrane protein.

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.