Anti-PDGFC Antibody Picoband®

About this product

Boster Bio Anti-PDGFC Antibody Picoband® catalog # A03092-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A03092-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Platelet-derived growth factor C, also known as PDGF-C, is a 345-amino acid protein that in humans is encoded by the PDGFC gene. The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines. This gene product appears to form only homodimers. It differs from the platelet-derived growth factor alpha and beta polypeptides in having an unusual N-terminal domain, the CUB domain. Alternatively spliced transcript variants have been found for this gene.

Synonyms

NADPH oxidase 5; NOX5

Gene Name

PDGFC

Gene ID

56034

UniProt

Q9NRA1

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human PDGFC recombinant protein (Position: L288-G345) .

Clonality

Polyclonal

Tissue Specificity

Mainly expressed in pachytene spermatocytes of testis and in lymphocyte-rich areas of spleen and lymph nodes. Isoform v1 is expressed in spleen. Isoform v2 is expressed in testis. Also detected in ovary, placenta, pancreas, cardiac fibroblasts. Expressed in B-cells and prostate malignant cells. Isoform v1 and isoform v3 are expressed in epithelial colorectal adenocarcinoma cells. Isoform v2 and isoform v4 are expressed in endothelial cells. Isoform v1, isoform v2, isoform v3 and isoform v4 are expressed in pulmonary artery smooth muscle cells. Isoform v2 and isoform v5 are expressed in microvascular endothelial cells (at protein level) .

Applications

WB, Flow Cytometry, ELISA

Field of Research

Apoptosis, Associated Proteins, Cell Biology, Cell Cycle, Cell Differentiation, Channels, Intracellular, Metabolism, Oxidative Stress, Pathways and Processes, Plasma Membrane, Redox Metabolism, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Calcium-dependent NADPH oxidase that generates superoxide. Also functions as a calcium-dependent proton channel and may regulate redox-dependent processes in lymphocytes and spermatozoa. May play a role in cell growth and apoptosis. Isoform v2 and isoform v5 are involved in endothelial generation of reactive oxygen species (ROS), proliferation and angiogenesis and contribute to endothelial response to thrombin.

References & Citations

1. Ding, H., Wu, X., Bostrom, H., Kim, I., Wong, N., Tsoi, B., O'Rourke, M., Koh, G. Y., Soriano, P., Betsholtz, C., Hart, T. C., Marazita, M. L., Field, L. L., Tam, P. P. L., Nagy, A. A specific requirement for PDGF-C in palate formation and PDGFR-alpha signaling. Nature Genet. 36: 1111-1116, 2004. 2. Fredriksson, L., Li, H., Fieber, C., Li, X., Eriksson, U. Tissue plasminogen activator is a potent activator of PDGF-CC. EMBO J. 23: 3793-3802, 2004. 3. Gilbertson, D. G., Duff, M. E., West, J. W., Kelly, J. D., Sheppard, P. O., Hofstrand, P. D., Gao, Z., Shoemaker, K., Bukowski, T. R., Moore, M., Feldhaus, A. L., Humes, J. M., Palmer, T. E., Hart, C. E. Platelet-derived growth factor C (PDGF-C), a novel growth factor that binds PDGF alpha and beta receptor. J. Biol. Chem. 276: 27406-27414, 2001.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

64406 MW

Observed Molecular Weight

39 kDa

Applications Notes

6

Gene Name Synonym

NADPH oxidase 5

Subcellular Location

Membrane; Multi-pass membrane protein.

Protein Name

Transcription factor E2F3

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.