Anti-PTPN22 Antibody Picoband®

About this product

Boster Bio Anti-PTPN22 Antibody Picoband® catalog # A00581-3. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00581-3

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Protein tyrosine phosphatase, non-receptor type 22 (lymphoid), also known as PTPN22, is a protein that in humans is encoded by the PTPN22 gene. It is mapped to 1p13.2. This gene encodes of member of the non-receptor class 4 subfamily of the protein-tyrosine phosphatase family. The encoded protein is a lymphoid-specific intracellular phosphatase that associates with the molecular adapter protein CBL and may be involved in regulating CBL function in the T-cell receptor signaling pathway. Mutations in this gene may be associated with a range of autoimmune disorders including Type 1 Diabetes, rheumatoid arthritis, systemic lupus erythematosus and Graves' disease. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Synonyms

Tyrosine-protein phosphatase non-receptor type 22; Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP; Lymphoid phosphatase; LyP; PEST-domain phosphatase; PEP; PTPN22; PTPN8

Gene Name

PTPN22

Gene ID

26191

UniProt

Q9Y2R2

Host

Rabbit

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human PTPN22 recombinant protein (Position: M1-K42) .

Clonality

Polyclonal

Tissue Specificity

Spleen, thymus, lymph node and bone marrow.

Applications

WB, ICC, IF, ELISA

Field of Research

Protein Phosphorylation, Signal Transduction, Tyrosine Phosphatases

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Acts as negative regulator of T-cell receptor (TCR) signaling by dephosphorylation of the Src family kinases LCK and FYN, ITAMs of the TCRz/CD3 complex, as well as ZAP70, VAV, VCP and other key signaling molecules. Associates with and probably dephosphorylates CBL. Dephosphorylates LCK at its activating 'Tyr-394' residue. Dephosphorylates ZAP70 at its activating 'Tyr-493' residue. Dephosphorylates the immune system activator SKAP2. Positively regulates toll-like receptor (TLR) -induced type 1 interferon production. Promotes host antiviral responses mediated by type 1 interferon. Regulates NOD2-induced pro-inflammatory cytokine secretion and autophagy.

References & Citations

1. Kallberg, H., Padyukov, L., Plenge, R. M., Ronnelid, J., Gregersen, P. K., van der Helm-van Mil, A. H. M., Toes, R. E. M., Huizinga, T. W., Klareskog, L., Alfredsson, L. Gene-gene and gene-environment interactions involving HLA-DRB1, PTPN22, and smoking in two subsets of rheumatoid arthritis. Am. J. Hum. Genet. 80: 867-875, 2007. 2. Kawasaki, E., Awata, T., Ikegami, H., Kobayashi, T., Maruyama, T., Nakanishi, K., Shimada, A., Uga, M., Kurihara, S., Kawabata, Y., Tanaka, S., Kanazawa, Y., Lee, I., Eguchi, K., Japanese Study Group on Type 1 Diabetes Genetics. Systematic search for single nucleotide polymorphisms in a lymphoid tyrosine phosphatase gene (PTPN22) : association between a promoter polymorphism and type 1 diabetes in Asian populations. Am. J. Med. Genet. 140A: 586-593, 2006. Note: Erratum: Am. J. Med. Genet. 143A: 1812-1813, 2007. 3. Kyogoku, C., Langefeld, C. D., Ortmann, W. A., Lee, A., Selby, S., Carlton, V. E. H., Chang, M., Ramos, P., Baechler, E. C., Batliwalla, F. M., Novitzke, J., Williams, A. H., and 10 others. Genetic association of the R620W polymorphism of protein tyrosine phosphatase PTPN22 with human SLE. Am. J. Hum. Genet. 75: 504-507, 2004.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

86830 MW

Observed Molecular Weight

92 kDa

Gene Name Synonym

Protein tyrosine phosphatase non-receptor type 22

Subcellular Location

Cytoplasm.

Protein Name

Interferon regulatory factor 1

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.