Anti-KAT3B/p300/EP300 Antibody Picoband®

About this product

Boster Bio Anti-KAT3B/p300/EP300 Antibody Picoband® catalog # A00117. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00117

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

E1A binding protein p300 also known as EP300 or p300 is a protein that in humans is encoded by the EP300 gene. The EP300 gene is located on the long (q) arm of the human chromosome 22 at position 13.2. This protein regulates the activity of many genes in tissues throughout the body. It plays an essential role in regulating cell growth and division, prompting cells to mature and assume specialized functions (differentiate), and preventing the growth of cancerous tumors. The EP300 protein appears to be critical for normal development before and after birth. It carries out its function by activating transcription. In addition, the protein functions as histone acetyltransferase that regulates transcription via chromatin remodeling, and is important in the processes of cell proliferation and differentiation. EP300 also mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein.

Synonyms

GTPase HRas; H-Ras-1; Ha-Ras; Transforming protein p21; c-H-ras; p21ras; GTPase HRas, N-terminally processed; HRAS; HRAS1

Gene Name

EP300

Gene ID

2033

UniProt

Q09472

Host

Rabbit

Reactivity

Human, Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human KAT3B/p300/EP300, which shares 76.5% amino acid (aa) sequence identity with mouse EP300.

Clonality

Polyclonal

Tissue Specificity

Widely expressed. .

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Associated Proteins, Cancer, Cancer Susceptibility, Cell Biology, Cell Cycle, Cell Differentiation, Epigenetics and Nuclear Signaling, G Protein Signaling, Intracellular, Nucleotide Messenger, Proto-Oncogenes, Ras Family, Second Messenger, Signal Transduction, Signaling Pathway, Small G Proteins, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. .

References & Citations

1. Eckner R, Ewen ME, Newsome D, Gerdes M, DeCaprio JA, Lawrence JB, Livingston DM (April 1994) . Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with properties of a transcriptional adaptor. Genes Dev. 8 (8) : 869–84. 2. Ogryzko VV et al. The transcriptional coactivators p300 and CBP are histone acetyltransferases. Cell. 1996 87 (5) :953-9.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

21298 MW

Observed Molecular Weight

264-300 kDa

Applications Notes

6

Gene Name Synonym

GTPase HRas

Subcellular Location

Cell membrane. Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus. Golgi apparatus membrane; Lipid-anchor. The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form (By similarity) . Shuttles between the plasma membrane and the Golgi apparatus. .

Protein Name

GTPase Hras

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.