Anti-p38 alpha/MAPK14 Antibody Picoband®

About this product

Boster Bio Anti-p38 alpha/MAPK14 Antibody Picoband® catalog # A00176-2. Tested in Flow Cytometry, IP, ICC/IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00176-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mitogen-activated protein kinase 14, also called p38-α, is an enzyme that in humans is encoded by the MAPK14 gene. The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Synonyms

Mannose-6-phosphate isomerase;5.3.1.8; Phosphohexomutase; Phosphomannose isomerase; PMI; MPI; PMI1

Gene Name

MAPK14

Gene ID

1432

UniProt

Q16539

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human p38 alpha/MAPK14, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Tissue Specificity

Expressed in all tissues, but more abundant in heart, brain and skeletal muscle.

Applications

Flow Cytometry, IP, ICC/IF, IHC, WB

Field of Research

Energy Metabolism, Energy Transfer Pathways, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.

References & Citations

1. Adams, R. H., Porras, A., Alonso, G., Jones, M., Vintersten, K., Panelli, S., Valladares, A., Perez, L., Klein, R., Nebreda, A. R. Essential role of p38-alpha MAP kinase in placental but not embryonic cardiovascular development. Molec. Cell 6: 109-116, 2000. 2. Agrawal, A., Lingappa, J., Leppla, S. H., Agrawal, S., Jabbar, A., Quinn, C., Pulendran, B. Impairment of dendritic cells and adaptive immunity by anthrax lethal toxin. Nature 424: 329-334, 2003. 3. Anhalt, G. J., Labib, R. S., Voorhees, J. J., Beals, T. F., Diaz, L. A. Induction of pemphigus in neonatal mice by passive transfer of IgG from patients with the disease. New Eng. J. Med. 306: 1189-1196, 1982.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

41 kDa

Observed Molecular Weight

38 kDa

Applications Notes

6

Gene Name Synonym

Mannose-6-phosphate isomerase

Subcellular Location

Cytoplasm .

Protein Name

Mannose-6-phosphate isomerase

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.