Anti-BAP1 Antibody Picoband®

About this product

Boster Bio Anti-BAP1 Antibody Picoband® catalog # A00345-2. Tested in ELISA, Flow Cytometry, ICC/IF, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00345-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

BAP1, also known as BRCA1-associated protein-1, contains an acidic region, a highly charged C-terminal region, and 2 putative nuclear localization signals. BAP1 is a novel ubiquitin hydrolase which binds to the BRCA1 RING finger and enhances BRCA1-mediated cell growth suppression. BAP1 is expressed as a 4-kb mRNA in all human tissues, and mapped to 3p21.3.

Synonyms

Ubiquitin carboxyl-terminal hydrolase BAP1; BRCA1-associated protein 1; Cerebral protein 6; BAP1; KIAA0272; hucep-6

Gene Name

BAP1

Gene ID

8314

UniProt

Q92560

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human BAP1 recombinant protein (Position: E7-Q702) .

Clonality

Polyclonal

Tissue Specificity

Highly expressed in testis, placenta and ovary. Expressed in breast.

Applications

WB, ICC/IF, Flow Cytometry, ELISA

Field of Research

Cancer, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Deubiquitination, Epigenetics and Nuclear Signaling, Ubiquitin & Ubiquitin Like Modifiers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1) . Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'- linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Able to mediate autodeubiquitination via intramolecular interactions to couteract monoubiquitination at the nuclear localization signal (NLS), thereby protecting it from cytoplasmic sequestration (PubMed:24703950) . Acts as a tumor suppressor.

References & Citations

1. Jensen, D. E.; Proctor, M.; Marquis, S. T.; Gardner, H. P.; Ha, S. I.; Chodosh, L. A.; Ishov, A. M.; Tommerup, N.; Vissing, H.; Sekido, Y.; Minna, J.; Borodovsky, A.; Schultz, D. C.; Wilkinson, K. D.; Maul, G. G.; Barlev, N.; Berger, S. L.; Prendergast, G. C.; Rauscher, F. J., III : BAP1: a novel ubiquitin hydrolase which binds to the BRCA1 RING finger and enhances BRCA1-mediated cell growth suppression. Oncogene 16: 1097-1112, 1998.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

80 kDa

Observed Molecular Weight

95-100 kDa

Applications Notes

6

Gene Name Synonym

BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase)

Subcellular Location

Cytoplasm.

Protein Name

Beta-nerve growth factor

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.