Anti-Dectin-1/Clec7a Antibody Picoband®

About this product

Boster Bio Anti-Dectin-1/Clec7a Antibody Picoband® catalog # A02731-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02731-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

C-type lectin domain family 7 member A or Dectin-1 is a protein that in humans is encoded by the CLEC7A gene. This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. The encoded glycoprotein is a small type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif. It functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region.

Synonyms

Nuclear factor of activated T-cells, cytoplasmic 3; NF-ATc3; NFATc3; NFATx; T-cell transcription factor NFAT4; NF-AT4; NFATC3; NFAT4

Gene Name

Clec7a

Gene ID

56644

UniProt

Q6QLQ4

Host

Rabbit

Reactivity

Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived mouse Dectin-1/Clec7a recombinant protein (Position: Q17-L244) .

Clonality

Polyclonal

Tissue Specificity

Isoform 1 is predominantly expressed in thymus and is also found in peripheral blood leukocytes and kidney. Isoform 2 is predominantly expressed in skeletal muscle and is also found in thymus, kidney, testis, spleen, prostate, ovary, small intestine, heart, placenta and pancreas. Isoform 3 is expressed in thymus and kidney. Isoform 4 is expressed in thymus and skeletal muscle.

Applications

WB, IHC, Flow Cytometry, ELISA

Field of Research

Epigenetics and Nuclear Signaling, Nuclear Signaling, Nuclear Signaling Pathways, Signal Transduction, Signaling Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Acts as a regulator of transcriptional activation. Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2.

References & Citations

1. Ariizumi, K., Shen, G.-L., Shikano, S., Xu, S., Ritter, R., III, Kumamoto, T., Edelbaum, D., Morita, A., Bergstresser, P. R., Takashima, A. Identification of a novel, dendritic cell-associated molecule, dectin-1, by subtractive cDNA cloning. J. Biol. Chem. 275: 20157-20167, 2000. [ 2. Brown, G. D., Gordon, S. A new receptor for beta-glucans. Nature 413: 36-37, 2001. 3. Brown, G. D., Herre, J., Williams, D. L., Willment, J. A., Marshall, A. S. J., Gordon, S. Dectin-1 mediates the biological effects of beta-glucans. J. Exp. Med. 197: 1119-1124, 2003.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

33 kDa

Applications Notes

6

Gene Name Synonym

Nuclear factor of activated T-cells 3

Subcellular Location

Cytoplasm

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.