Anti-Cathepsin L/MEP/CTSL Antibody Picoband®

About this product

Boster Bio Anti-Cathepsin L/MEP/CTSL Antibody Picoband® catalog # A01589-2. Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01589-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. Additionally, this protein cleaves the S1 subunit of the SARS-CoV-2 spike protein, which is necessary for entry of the virus into the cell.

Synonyms

Neutrophil cytosol factor 1; NCF-1; 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; SH3 and PX domain-containing protein 1A; p47-phox; NCF1; NOXO2; SH3PXD1A

Gene Name

CTSL

Gene ID

1514

UniProt

P07711

Host

Rabbit

Reactivity

Human, Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Cathepsin L/MEP/CTSL, which shares 61.1% amino acid (aa) sequence identity with rat CTSL.

Clonality

Polyclonal

Tissue Specificity

Detected in peripheral blood monocytes and neutrophils (at protein level) .

Applications

WB

Field of Research

Cancer, Cancer Metabolism, Energy Metabolism, Energy Transfer Pathways, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production) .

References & Citations

1. Abudula, A., Rommerskirch, W., Weber, E., Gunther, D., Wiederanders, B. Splice variants of human cathepsin L mRNA show different expression rates. Biol. Chem. 382: 1583-1591, 2001. 2. Arora, S., Chauhan, S. S. Identification and characterization of a novel human cathepsin L splice variant. Gene 293: 123-131, 2002. 3. Bakhshi, R., Goel, A., Seth, P., Chhikara, P., Chauhan, S. S. Cloning and characterization of human cathepsin L promoter. Gene 275: 93-101, 2001.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

34140 MW

Observed Molecular Weight

37 kDa

Gene Name Synonym

Neutrophil cytosolic factor 1

Subcellular Location

Cytoplasm, cytosol.

Protein Name

Growth/differentiation factor 15

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.