Anti-Aromatase/Cyp19a1 Antibody Picoband®

About this product

Boster Bio Anti-Aromatase/Cyp19a1 Antibody Picoband® catalog # A00071-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00071-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CYP19A1, also called Aromatase, is an enzyme responsible for a key step in the biosynthesis of estrogens. It is a member of the cytochrome P450 superfamily, which are monooxygenases that catalyze many reactions involved in steroidogenesis. In particular, aromatase is responsible for the aromatization of androgens into estrogens. The CYP19 gene spans at least 70 kb of genomic DNA and contains 10 exons. By in situ hybridization, the ARO gene is mapped to 15q21.1. The aromatase enzyme can be found in many tissues including gonads, brain, adipose tissue, placenta, blood vessels, skin, bone, and endometrium, as well as in tissue of endometriosis, uterine fibroids, breast cancer, and endometrial cancer. It is an important factor in sexual development. Some bodybuilders taking steroids also take antiaromatase supplements to prevent excess testosterone conversion into estrogens, which can cause gynecomastia.

Synonyms

Aromatase; CYPXIX; Cytochrome P-450AROM; Cytochrome P450 19A1; Estrogen synthase; CYP19A1; ARO1; CYAR; CYP19

Gene Name

Cyp19a1

Gene ID

13075

UniProt

P28649

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived mouse Aromatase/Cyp19a1 recombinant protein (Position: N75-Q428) .

Clonality

Polyclonal

Tissue Specificity

Widely expressed, including in adult and fetal brain, placenta, skin fibroblasts, adipose tissue and gonads.

Applications

WB, ELISA

Field of Research

Cancer, Cardiovascular, Cytochromes, Drug Metabolism, Growth Factors/Hormones, Lipases, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Mitochondrial Metabolism, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Catalyzes the formation of aromatic C18 estrogens from C19 androgens.

References & Citations

1. Chen, S., Besman, M. J., Sparkes, R. S., Zollman, S., Klisak, I., Mohandas, T., Hall, P. F., Shively, J. E.Human aromatase: cDNA cloning, Southern blot analysis, and assignment of the gene to chromosome 15.DNA 7: 27-38, 1988. 2. Harada, N., Ogawa, H., Shozu, M., Yamada, K., Suhara, K., Nishida, E., Takagi, Y.Biochemical and molecular genetic analyses on placental aromatase (P-450-AROM) deficiency.J. Biol. Chem. 267: 4781-4785, 1992. 3. Zhou, D., Pompon, D., Chen, S.Structure-function studies of human aromatase by site-ed mutagenesis: kinetic properties of mutants pro308-to-phe, tyr361-to-phe, tyr361-to-leu, and phe406-to-arg.Proc. Nat. Acad. Sci. 88: 410-414, 1991.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

49-58 kDa

Gene Name Synonym

Cytochrome P450, family 19, subfamily A, polypeptide 1

Subcellular Location

Membrane. Peripheral membrane protein.

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.