Anti-YWHAE Antibody Picoband® (monoclonal, 3G11G2)

About this product

Boster Bio Anti-YWHAE Antibody Picoband® (monoclonal, 3G11G2) catalog # M01687-2. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M01687-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

14-3-3 protein epsilon is a protein that in humans is encoded by the YWHAE gene. This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene.

Synonyms

A32 antigen antibody; CD 146 antibody; CD146 antibody; CD146 antigen antibody; Cell surface glycoprotein MUC18 antibody; Cell surface glycoprotein P1H12 antibody; Gicerin antibody; MCAM antibody; Melanoma adhesion molecule antibody; Melanoma associated antigen A32 antibody; Melanoma associated antigen MUC18 antibody; Melanoma associated glycoprotein MUC18 antibody; Melanoma cell adhesion molecule antibody; Melanoma-associated antigen A32 antibody; Melanoma-associated antigen MUC18 antibody; MelCAM antibody; MUC 18 antibody; MUC18 antibody; MUC18_HUMAN antibody; S endo 1 antibody; S endo 1 endothelial associated antigen antibody; S-endo 1 endothelial-associated antigen antibody; Sendo 1 endothelial associated antigen antibody; Sendo1 antibody

Gene Name

YWHAE

Gene ID

7531

UniProt

P62258

Host

Mouse

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human YWHAE recombinant protein (Position: M1-Q255) .

Clonality

Monoclonal

Clone

Clone: 3G11G2

Tissue Specificity

Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis.

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Actin Binding Proteins, Actin, etc., Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.

References & Citations

1. Bi, W., Sapir, T., Shchelochkov, O. A., Zhang, F., Withers, M. A., Hunter, J. V., Levy, T., Shinder, V., Peiffer, D. A., Gunderson, K. L., Nezarati, M. M., Shotts, V. A., and 13 others. Increased LIS1 expression affects human and mouse brain development. Nature Genet. 41: 168-177, 2009. 2. Cardoso, C., Leventer, R. J., Ward, H. L., Toyo-oka, K., Chung, J., Gross, A., Martin, C. L., Allanson, J., Pilz, D. T., Olney, A. H., Mutchinick, O. M., Hirotsune, S., Wynshaw-Boris, A., Dobyns, W. B., Ledbetter, D. H. Refinement of a 400-kb critical region allows genotypic differentiation between isolated lissencephaly, Miller-Dieker syndrome, and other phenotypes secondary to deletions of 17p13.3. Am. J. Hum. Genet. 72: 918-930, 2003. 3. Chong, S. S., Tanigami, A., Roschke, A. V., Ledbetter, D. H. 14-3-3-epsilon has no homology to LIS1 and lies telomeric to it on chromosome 17p13.3 outside the Miller-Dieker syndrome chromosome region. Genome Res. 6: 735-741, 1996.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

29 kDa

Applications Notes

6

Gene Name Synonym

Melanoma cell adhesion molecule

Subcellular Location

Membrane. Single-pass type I membrane protein.

Protein Name

Activating transcription factor 1

Isotype

Mouse IgG2b

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.