Anti-CD147/Emmprin Antibody Picoband® (monoclonal, 3B13G7)

About this product

Boster Bio Anti-CD147/Emmprin Antibody Picoband® (monoclonal, 3B13G7) catalog # M00248-4. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M00248-4

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Emmprin, extracellular matrix metalloproteinase inducer, also known as Emmprin (BSG) or cluster of differentiation 147 (CD147) is a protein that in humans is encoded by the Emmprin gene. The human BSG gene is mapped to 19p13.3. This protein is a determinant for the Ok blood group system. BSG has been shown to be an essential receptor on red blood cells for the malaria parasite. It is a member of the immunoglobulin superfamily, with a structure related to the putative primordial form of the family. As members of the immunoglobulin superfamily, it plays fundamental roles in intercellular recognition involved in various immunologic phenomena, differentiation, and development. BSG is thought also to play a role in intercellular recognition. It also regulates several distinct functions, such as spermatogenesis, expression of the monocarboxylate transporter and the responsiveness of lymphocytes. BSG is a type I integral membrane receptor that has many ligands, including the cyclophilin (CyP) proteins Cyp-A and CyP-B and certain integrins. It is expressed by many cell types, including epithelial cells, endothelial cells and leukocytes.

Synonyms

Tuberin; Tuberous sclerosis 2 protein; TSC2; TSC4

Gene Name

BSG

Gene ID

682

UniProt

P35613

Host

Mouse

Reactivity

Human, Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human CD147/Emmprin recombinant protein (Position: E138-A323) . Human CD147/Emmprin shares 51.1% and 51.9% amino acid (aa) sequence identity with mouse and rat CD147/Emmprin, respectively.

Clonality

Monoclonal

Clone

Clone: 3B13G7

Tissue Specificity

Liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta.

Applications

WB, IHC, IF

Field of Research

Calcium Channels, Calcium Signaling, Cancer, Cancer Metabolism, Energy Transfer Pathways, Integration Of Energy, Integration Of Energy Metabolism, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Neuroscience, Neurotransmission, Pathways and Processes, Signal Transduction, Signaling Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

In complex with TSC1, this tumor suppressor inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling (PubMed:12271141, PubMed:28215400) . Acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a activator of the protein kinase activity of mTORC1 (PubMed:15340059) . May also play a role in microtubule-mediated protein transport (By similarity) . Also stimulates the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 (By similarity) .

References & Citations

1. Guo, H., Majmudar, G., Jensen, T. C., Biswas, C., Toole, B. P., Gordon, M. K.Characterization of the gene for human EMMPRIN, a tumor cell surface inducer of matrix metalloproteinases.Gene 220: 99-108, 1998. 2. Zhou, S., Zhou, H., Walian, P. J., Jap, B. K.CD147 is a regulatory subunit of the gamma-secretase complex in Alzheimer's disease amyloid beta-peptide production.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

35-60 kDa

Gene Name Synonym

Tuberous sclerosis 2

Subcellular Location

Cytoplasm; Membrane; Peripheral membrane protein

Protein Name

Glial fibrillary acidic protein

Isotype

Mouse IgG1

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.