Anti-PDIA6 Antibody Picoband® (monoclonal, 3H5E7)

About this product

Boster Bio Anti-PDIA6 Antibody Picoband® (monoclonal, 3H5E7) catalog # M03813-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M03813-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, two catalytically active thioredoxin (TRX) domains, a TRX-like domain, and a C-terminal ER-retention sequence. This protein inhibits the aggregation of misfolded proteins and exhibits both isomerase and chaperone activity. Alternative splicing results in multiple transcript variants encoding different isoforms.

Synonyms

Eukaryotic translation initiation factor 6; eIF-6; B (2) GCN homolog; B4 integrin interactor; CAB; p27 (BBP) ; EIF6; EIF3A; ITGB4BP; OK/SW-cl.27

Gene Name

PDIA6

Gene ID

10130

UniProt

Q15084

Host

Mouse

Reactivity

Human, Monkey

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human PDIA6 recombinant protein (Position: L20-L440) .

Clonality

Monoclonal

Clone

Clone: 3H5E7

Tissue Specificity

Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle.

Applications

WB, IHC, ICC, IF, Flow Cytometry

Field of Research

Cell Adhesion Proteins, Epigenetics and Nuclear Signaling, Integrins, Mediator Complex, Neuroscience, Receptors, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Behaves as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (RACK1) -dependent protein kinase C activity. In tissues responsive to insulin, controls fatty acid synthesis and glycolysis by exerting translational control of adipogenic transcription factors such as CEBPB, CEBPD and ATF4 that have G/C rich or uORF in their 5'UTR. Required for ROS-dependent megakaryocyte maturation and platelets formation, controls the expression of mitochondrial respiratory chain genes involved in reactive oxygen species (ROS) synthesis. Involved in miRNA-mediated gene silencing by the RNA-induced silencing complex (RISC) . Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC. Modulates cell cycle progression and global translation of pre-B cells, its activation seems to be rate-limiting in tumorigenesis and tumor growth.

References & Citations

1. Chaudhuri, M. M., Tonin, P. N., Lewis, W. H., Srinivasan, P. R. The gene for a novel protein, a member of the protein disulphide isomerase/Form I phosphoinositide-specific phospholipase C family, is amplified in hydroxyurea-resistant cells. Biochem. J. 281: 645-650, 1992. 2. Hayano, T., Kikuchi, M. Cloning and sequencing of the cDNA encoding human P5. Gene 164: 377-378, 1995. 3. Hoshijima, K., Metherall, J. E., Grunwald, D. J. A protein disulfide isomerase expressed in the embryonic midline is required for left/right asymmetries. Genes Dev. 16: 2518-2529, 2002.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

48 kDa

Applications Notes

6

Gene Name Synonym

Eukaryotic translation initiation factor 6

Subcellular Location

Nucleolus. Cytoplasm

Protein Name

Mediator of RNA polymerase II transcription subunit 15

Isotype

Mouse IgG2b

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.