Anti-ACAT2 Antibody Picoband®

About this product

Boster Bio Anti-ACAT2 Antibody Picoband® catalog # A03245-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A03245-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Acetyl-CoA acetyltransferase, cytosolic, also known as cytosolic acetoacetyl-CoA thiolase, is an enzyme that in humans is encoded by the ACAT2 (acetyl-Coenzyme A acetyltransferase 2) gene. The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Synonyms

Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; CaMK-II subunit alpha; CAMK2A; CAMKA; KIAA0968

Gene Name

ACAT2

Gene ID

39

UniProt

Q9BWD1

Host

Rabbit

Reactivity

Human, Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human ACAT2 recombinant protein (Position: Q31-E397) .

Clonality

Polyclonal

Tissue Specificity

High levels found in the brain, heart and lung while lower levels are seen in the liver, kidney and skeletal muscle.

Applications

WB, Flow Cytometry, ELISA

Field of Research

Calcium Signaling, Calmodulin Pathway, Cardiovascular, Hypertrophy, Neuroscience, Neurotransmission, Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction, Signaling Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca2+/calmodulin-binding and autophosphorylation, and is involved in synaptic plasticity, neurotransmitter release and long-term potentiation. Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission. Regulates dendritic spine development. Also regulates the migration of developing neurons. Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity.

References & Citations

1. Ashworth, A. Two acetyl-CoA acetyltransferase genes located in the t-complex region of mouse chromosome 17 partially overlap the Tcp-1 and Tcp-1x genes. Genomics 18: 195-198, 1993. 2. de Groot, C. J., Luit-de Haan, G., Hulstaert, C. E., Hoomes, F. A. A patient with severe neurologic symptoms and acetoacetyl-CoA thiolase deficiency. Pediat. Res. 11: 1112-1116, 1977. 3. Laudet, V., Begue, A., Henry-Duthoit, C., Joubel, A., Martin, P., Stehelin, D., Saule, S. Genomic organization of the human thyroid hormone alpha (c-erbA-1) gene. Nucleic Acids Res. 19: 1105-1112, 1991.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

41 kDa

Applications Notes

6

Gene Name Synonym

Calcium/calmodulin dependent protein kinase II alpha

Subcellular Location

Synapse. Postsynaptic density. Dendritic spine. Dendrite.

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.