- Catalog number
- A00280-2
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CX3C chemokine receptor 1 (CX3CR1) also known as the fractalkine receptor or G-protein coupled receptor 13 (GPR13) is a protein that in humans is encoded by the CX3CR1 gene. Fractalkine is a transmembrane protein and chemokine involved in the adhesion and migration of leukocytes. The protein encoded by this gene is a receptor for fractalkine. The encoded protein also is a coreceptor for HIV-1, and some variations in this gene lead to increased susceptibility to HIV-1 infection and rapid progression to AIDS. Four transcript variants encoding two different isoforms have been found for this gene.
- Synonyms
- Merlin; Moesin-ezrin-radixin-like protein; Neurofibromin-2; Schwannomerlin; Schwannomin; NF2; SCH
- Gene Name
- CX3CR1
- Gene ID
- 1524
- UniProt
- P49238
- Host
- Rabbit
- Reactivity
- Human
- Cross Reactivity
- No cross-reactivity with other proteins.
- Immunogen
- E.coli-derived human CX3CR1 recombinant protein (Position: M1-H345) .
- Clonality
- Polyclonal
- Tissue Specificity
- Widely expressed. Isoform 1 and isoform 3 are predominant. Isoform 4, isoform 5 and isoform 6 are expressed moderately. Isoform 8 is found at low frequency. Isoform 7, isoform 9 and isoform 10 are not expressed in adult tissues, with the exception of adult retina expressing isoform 10. Isoform 9 is faintly expressed in fetal brain, heart, lung, skeletal muscle and spleen. Fetal thymus expresses isoforms 1, 7, 9 and 10 at similar levels.
- Applications
- ELISA, Flow Cytometry, IHC
- Field of Research
- Calcium Signaling, Cancer, Cardiovascular, Endocrine System, Fatty Acid Oxidation, Fatty Acids, Lipid and Lipoprotein Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Neurology Process, Neuroscience, Obesity, Pathways and Processes, Redox Metabolism, Signal Transduction, Signaling Pathway
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Function
- Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex.
- References & Citations
- 1. Combadiere, C., Feumi, C., Raoul, W., Keller, N., Rodero, M., Pezard, A., Lavalette, S., Houssier, M., Jonet, L., Picard, E., Debre, P., Sirinyan, M., Deterre, P., Ferroukhi, T., Cohen, S.-Y., Chauvaud, D., Jeanny, J.-C., Chemtob, S., Behar-Cohen, F., Sennlaub, F. CX3CR1-dependent subretinal microglia cell accumulation is associated with cardinal features of age-related macular degeneration. J. Clin. Invest. 117: 2920-2928, 2007. 2. Faure, S., Meyer, L., Costagliola, D., Vaneensberghe, C., Genin, E., Autran, B., French ALT and IMMUNOCO Study Groups, Delfraisay, J.-F., SEROCO Study Group, McDermott, D. H., Murphy, P. M., Debre, P., Theodorou, I., Combadiere, C. Rapid progression to AIDS in HIV+ individuals with a structural variant of the chemokine receptor CX (3) CR1. Science 287: 2274-2277, 2000. 3. Feldman, G. J., Parvizi, J., Levenstien, M., Scott, K., Erickson, J. A., Fortina, P., Devoto, M., Peters, C. L. Developmental dysplasia of the hip: linkage mapping and whole exome sequencing identify a shared variant in CX3CR1 in all affected members of a large multigeneration family. J. Bone Miner. Res. 28: 2540-2549, 2013.
- Storage Conditions
- At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
- Calculated Molecular Weight
- 68762 MW
- Observed Molecular Weight
- 69 kDa
- Applications Notes
- 6
- Gene Name Synonym
- Neurofibromin 2
- Subcellular Location
- Filopodium membrane. Peripheral membrane protein. Cytoplasmic side. Ruffle membrane. Peripheral membrane protein. Cytoplasmic side. Nucleus.
- Protein Name
- Aryl hydrocarbon receptor nuclear translocator-like protein 1
- Isotype
- Rabbit IgG
- Contents
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
