Anti-DNMT3B Antibody Picoband®

About this product

Boster Bio Anti-DNMT3B Antibody Picoband® catalog # A00319-4. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Monkey, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00319-4

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

DNA (cytosine-5-) -methyltransferase 3 beta, is an enzyme that in humans in encoded by the DNMT3B gene. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined.

Synonyms

Copper chaperone for superoxide dismutase; Superoxide dismutase copper chaperone; CCS

Gene Name

DNMT3B

Gene ID

1789

UniProt

Q9UBC3

Host

Rabbit

Reactivity

Human, Monkey, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human DNMT3B recombinant protein (Position: R17-R572) .

Clonality

Polyclonal

Tissue Specificity

Ubiquitous.

Applications

ELISA, Flow Cytometry, WB

Field of Research

Cancer, Cardiovascular, Metabolism, Neuroscience, Neurotransmission, Plasma Membrane, Potassium Channels, Receptors / Channels, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Delivers copper to copper zinc superoxide dismutase (SOD1) .

References & Citations

1. Balada, E., Ordi-Ros, J., Serrano-Acedo, S., Martinez-Lostao, L., Rosa-Leyva, M., Vilardell-Tarres, M. Transcript levels of DNA methyltransferases DNMT1, DNMT3A and DNMT3B in CD4+ T cells from patients with systemic lupus erythematosus. Immunology 124: 339-347, 2008. 2. Barau, J., Teissandier, A., Zamudio, N., Roy, S., Nalesso, V., Herault, Y., Guillou, F., Bourc'his, D. The DNA methyltransferase DNMT3C protects male germ cells from transposon activity. Science 354: 909-912, 2016. 3. Beaulieu, N., Morin, S., Chute, I. C., Robert, M.-F., Nguyen, H., MacLeod, A. R. An essential role for DNA methyltransferase DNMT3B in cancer cell survival. J. Biol. Chem. 277: 28176-28181, 2002.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

29041 MW

Observed Molecular Weight

96 kDa

Applications Notes

6

Gene Name Synonym

Copper chaperone for superoxide dismutase

Subcellular Location

Cytoplasm .

Protein Name

Copper chaperone for superoxide dismutase

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.