Anti-TRPV1 Antibody Picoband®

About this product

Boster Bio Anti-TRPV1 Antibody Picoband® catalog # A00128-4. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00128-4

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

The transient receptor potential cation channel, subfamily V, member 1 (TRPV1), also known as the capsaicin receptor is a protein which in humans is encoded by the TRPV1 gene. TRPV1 (also called Vanilloid receptor type 1) is a ligand-gated nonselective cation channel that is considered to be an important integrator of various pain stimuli such as endogenous lipids, capsaicin, heat, and low pH. In addition to expression in primary afferents, TRPV1 is also expressed in the CNS. Cui M et al. (2006) demonstrate that TRPV1 receptors in the CNS plays an important role in pain mediated by central sensitization. And the significant CNS penetration is necessary for a TRPV1 antagonist to produce broad-spectrum analgesia. And TRPV1 also participates in normal bladder function and is essential for normal mechanically evoked purinergic signaling by the urothelium.

Synonyms

Proliferating cell nuclear antigen; PCNA; Cyclin; PCNA

Gene Name

TRPV1

Gene ID

7442

UniProt

Q8NER1

Host

Rabbit

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human TRPV1 recombinant protein (Position: E107-A800) .

Clonality

Polyclonal

Applications

WB, ICC, IF, Flow Cytometry, ELISA

Field of Research

Angiogenesis, Cancer, Cardiovascular, Developmental Biology, Growth Factors, Growth Factors/Hormones, Invasion/Microenvironment, Neural Stem Cells, Neurogenesis, Neurology Process, Neuroscience, Organogenesis, Signal Transduction, Stem Cells

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902) . Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (PubMed:24695737) .

References & Citations

1. Bartoshuk, L. M., Duffy, V. B., Miller, I. J. PTC/PROP tasting: anatomy, psychophysics, and sex effects. Physiol. Behav. 56: 1165-1171, 1994. Note: Erratum: Physiol. Behav. 58: 203 only, 1995. 2. Basu, S., Srivastava, P. Immunological role of neuronal receptor vanilloid receptor 1 expressed on dendritic cells. Proc. Nat. Acad. Sci. 102: 5120-5125, 2005. 3. Bhave, G., Zhu, W., Wang, H., Brasier, D. J., Oxford, G. S., Gereau, R. W., IV. cAMP-dependent protein kinase regulates desensitization of the capsaicin receptor (VR1) by phosphorylation. Neuron 35: 721-731, 2002.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

95 kDa

Applications Notes

6

Gene Name Synonym

Proliferating cell nuclear antigen

Subcellular Location

Nucleus. Secreted.

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.