Anti-Histone H1.0/H1F0 Antibody Picoband® (monoclonal, 5I3E6)

About this product

Boster Bio Anti-Histone H1.0/H1F0 Antibody Picoband® (monoclonal, 5I3E6) catalog # M08821-2. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M08821-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

H1 histone family, member 0is a member of thehistonefamily of nuclearproteinswhich are a component ofchromatin. In humans, this protein is encoded by theH1F0gene. It is mapped to 22q13.1. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family.

Synonyms

T-complex protein 1 subunit gamma; TCP-1-gamma; CCT-gamma; hTRiC5; CCT3; CCTG; TRIC5

Gene Name

H1F0

Gene ID

3005

UniProt

P07305

Host

Mouse

Reactivity

Human, Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Histone H1.0/H1F0 recombinant protein (Position: K20-K159) .

Clonality

Monoclonal

Clone

Clone: 5I3E6

Tissue Specificity

Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Applications

WB, IHC, Flow Cytometry

Field of Research

Chaperones, Protein Trafficking, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.

References & Citations

1. Doenecke D, Tönjes R (February 1986) . Differential distribution of lysine and arginine residues in the closely related histones H1 and H5. Analysis of a human H1 gene. Journal of Molecular Biology. 187 (3) : 461–4. 2. Albig W, Drabent B, Kunz J, Kalff-Suske M, Grzeschik KH, Doenecke D (June 1993) . All known human H1 histone genes except the H1 (0) gene are clustered on chromosome 6. Genomics. 16 (3) : 649–54.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

24 kDa

Applications Notes

6

Gene Name Synonym

Chaperonin containing TCP1 subunit 3

Subcellular Location

Cytoplasm.

Isotype

Mouse IgG2b

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.