Anti-CD20 Antibody Picoband® (monoclonal, 4D11)

About this product

Boster Bio Anti-CD20 Antibody Picoband® (monoclonal, 4D11) catalog # M03780-5. Tested in IF, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M03780-5

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD20, also known as MS4A1, is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. It is mapped to 11q12.2. This gene encodes a member of the membrane-spanning 4A gene family. The function of CD20 is to enable optimal B-cell immune response, specifically against T-independent antigens. It is suspected that CD20 acts as a calcium channel in the cell membrane. What’s more, this protein may be involved in the regulation of B-cell activation and proliferation.

Synonyms

Eukaryotic translation initiation factor 6; eIF-6; B (2) GCN homolog; B4 integrin interactor; CAB; p27 (BBP) ; EIF6; EIF3A; ITGB4BP; OK/SW-cl.27

Gene Name

MS4A1

Gene ID

931

UniProt

P11836

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human CD20 recombinant protein (Position: M1-D261) . Human CD20 shares 75% amino acid (aa) sequence identity with mouse CD20.

Clonality

Monoclonal

Clone

Clone: 4D11

Tissue Specificity

Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle.

Applications

WB, IHC, IF

Field of Research

Cell Adhesion Proteins, Epigenetics and Nuclear Signaling, Integrins, Mediator Complex, Neuroscience, Receptors, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Behaves as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (RACK1) -dependent protein kinase C activity. In tissues responsive to insulin, controls fatty acid synthesis and glycolysis by exerting translational control of adipogenic transcription factors such as CEBPB, CEBPD and ATF4 that have G/C rich or uORF in their 5'UTR. Required for ROS-dependent megakaryocyte maturation and platelets formation, controls the expression of mitochondrial respiratory chain genes involved in reactive oxygen species (ROS) synthesis. Involved in miRNA-mediated gene silencing by the RNA-induced silencing complex (RISC) . Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC. Modulates cell cycle progression and global translation of pre-B cells, its activation seems to be rate-limiting in tumorigenesis and tumor growth.

References & Citations

1. Cragg MS, Walshe CA, Ivanov AO, Glennie MJ (2005) . The biology of CD20 and its potential as a target for mAb therapy. Curr. Dir. Autoimmun. Current ions in Autoimmunity 8: 140–74. 2. Hardy, Richard (2008) . Chapter 7: B Lymphocyte Development and Biology. In Paul, William.Fundamental Immunology (Book) (6th ed.) . Philadelphia: Lippincott Williams & Wilkins. pp. 237–269. 3. Kuijpers TW, Bende RJ, Baars PA, Grummels A, Derks IA, Dolman KM, Beaumont T, Tedder TF, van Noesel CJ, Eldering E, van Lier RA (January 2010) . CD20 deficiency in humans results in impaired T cell-independent antibody responses. J. Clin. Invest. 120 (1) : 214–22.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

33 kDa

Gene Name Synonym

Eukaryotic translation initiation factor 6

Subcellular Location

Nucleolus. Cytoplasm

Protein Name

Mediator of RNA polymerase II transcription subunit 15

Isotype

Mouse IgG2b

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.