- Catalog number
- M00910-3
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Complement decay-accelerating factor, also known as CD55 or DAF, is a protein that, in humans, is encoded by the CD55 gene. This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM) . Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins.
- Synonyms
- ATP dependent helicase RENT1 antibody; ATP-dependent helicase RENT1 antibody; Delta helicase antibody; FLJ43809 antibody; FLJ46894 antibody; HUPF 1 antibody; hUpf1 antibody; KIAA0221 antibody; Nonsense mRNA reducing factor 1 antibody; NORF 1 antibody; NORF1 antibody; pNORF 1 antibody; pNORF1 antibody; Regulator of nonsense transcripts 1 antibody; RENT 1 antibody; RENT1 antibody; RENT1_HUMAN antibody; Smg 2 antibody; Smg 2 homolog nonsense mediated mRNA decay factor antibody; UP Frameshift 1 antibody; Up frameshift mutation 1 homolog (S. cerevisiae) antibody; Up frameshift mutation 1 homolog antibody; Up frameshift suppressor 1 homolog antibody; Up-frameshift suppressor 1 homolog antibody; UPF 1 antibody; UPF 1 regulator of nonsense transcripts homolog antibody; upf1 antibody; UPF1 regulator of nonsense transcripts homolog antibody; Yeast Upf1p homolog antibody
- Gene Name
- CD55
- Gene ID
- 1604
- UniProt
- P08174
- Host
- Mouse
- Reactivity
- Human
- Cross Reactivity
- No cross-reactivity with other proteins.
- Immunogen
- E.coli-derived human CD55 recombinant protein (Position: D35-K347) . Human CD55 shares 49.1% amino acid (aa) sequence identity with mouse CD55.
- Clonality
- Monoclonal
- Clone
- Clone: 9F3A1
- Tissue Specificity
- Ubiquitous.
- Applications
- WB, IHC, ICC, IF
- Field of Research
- Apoptosis, Cancer, Cardiovascular, G Protein Signaling, Heterotrimeric G Proteins, Neural Signal Transduction, Neurology Process, Neuroscience, Nucleotide Messenger, Second Messenger, Signal Transduction, Signaling Pathway, Small G Proteins
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Function
- RNA-dependent helicase and ATPase required for nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation; its phosphorylation appears to be a key step in NMD. Recruited by release factors to stalled ribosomes together with the SMG1C protein kinase complex to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) (located 50-55 or more nucleotides downstream from the termination codon) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Phosphorylated UPF1 is recognized by EST1B/SMG5, SMG6 and SMG7 which are thought to provide a link to the mRNA degradation machinery involving exonucleolytic and endonucleolytic pathways, and to serve as adapters to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation and allowing the recycling of NMD factors. UPF1 can also activate NMD without UPF2 or UPF3, and in the absence of the NMD-enhancing downstream EJC indicative for alternative NMD pathways. Plays a role in replication-dependent histone mRNA degradation at the end of phase S; the function is independent of UPF2. For the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. The ATPase activity of UPF1 is required for disassembly of mRNPs undergoing NMD. Essential for embryonic viability.
- References & Citations
- 1. Medof ME, Lublin DM, Holers VM, Ayers DJ, Getty RR, Leykam JF, Atkinson JP, Tykocinski ML (April 1987) . Cloning and characterization of cDNAs encoding the complete sequence of decay-accelerating factor of human complement”. Proc. Natl. Acad. Sci. U.S.A. 84 (7) : 2007–11.
- Storage Conditions
- At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
- Observed Molecular Weight
- 70-75 kDa
- Gene Name Synonym
- UPF1 regulator of nonsense transcripts homolog (yeast)
- Subcellular Location
- Nucleus. Cytoplasm. P-body.
- Isotype
- Mouse IgG2b
- Contents
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
