Anti-PC4/SUB1 Antibody Picoband® (monoclonal, 6B5B10)

About this product

Boster Bio Anti-PC4/SUB1 Antibody Picoband® (monoclonal, 6B5B10) catalog # M02698-2. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M02698-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Activated RNA polymerase II transcriptional coactivator p15, also known as positive cofactor 4 (PC4) or SUB1 homolog, is a protein that in humans is encoded by the SUB1 gene. This gene is mapped to 5p13.3. The transcriptional cofactor PC4 is an ancient single-strand DNA (ssDNA) -binding protein that has a homologue in bacteriophage T5 where it is likely the elusive replicative ssDNA-binding protein. The recombinant PC4 is shown to function identically to the native protein through its interaction with TAFs.

Synonyms

Alpha-actinin-3; Alpha-actinin skeletal muscle isoform 3; F-actin cross-linking protein; ACTN3

Gene Name

SUB1

Gene ID

10923

UniProt

P53999

Host

Mouse

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human PC4/SUB1 recombinant protein (Position: N62-L127) .

Clonality

Monoclonal

Clone

Clone: 6B5B10

Tissue Specificity

Expressed only in a subset of type 2 skeletal muscle fibers.

Applications

WB, IHC

Field of Research

Actin Binding Proteins, Actin, etc., Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

References & Citations

1. Entrez Gene SUB1: SUB1 homolog (S. cerevisiae) . 2. Kretzschmar M, Kaiser K, Lottspeich F, Meisterernst M (August 1994) . A novel mediator of class II gene transcription with homology to viral immediate-early transcriptional regulators. Cell 78 (3) : 525–34. 3. Ge H, Roeder RG (August 1994) . Purification, cloning, and characterization of a human coactivator, PC4, that mediates transcriptional activation of class II genes. Cell 78 (3) : 513–23.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

18 kDa

Gene Name Synonym

Actinin alpha 3 (gene/pseudogene)

Isotype

Mouse IgG2b

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.