Anti-CD28 Antibody Picoband®

About this product

Boster Bio Anti-CD28 Antibody Picoband® catalog # A00065-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00065-3

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Synonyms

Haptoglobin; Zonulin; HP

Gene Name

CD28

Gene ID

940

UniProt

P10747

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human CD28 recombinant protein (Position: Q15-D214) .

Clonality

Polyclonal

Tissue Specificity

Expressed by the liver and secreted in plasma.

Applications

WB, Flow Cytometry, ELISA

Field of Research

Tags & Cell Markers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway. Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.

References & Citations

1. Akieda, Y., Wakamatsu, E., Nakamura, T., Ishida, Y., Ogawa, S., Abe, R. Defects in regulatory T cells due to CD28 deficiency induce a qualitative change of allogeneic immune response in chronic graft-versus-host disease. J. Immun. 194: 4162-4174, 2015. 2. Andres, P. G., Howland, K. C., Nirula, A., Kane, L. P., Barron, L., Dresnek, D., Sadra, A., Imboden, J., Weiss, A., Abbas, A. K. Distinct regions in the CD28 cytoplasmic domain are required for T helper type 2 differentiation. Nature Immun. 5: 435-442, 2004. 3. Aruffo, A., Seed, B. Molecular cloning of a CD28 cDNA by a high-efficiency COS cell expression system. Proc. Nat. Acad. Sci. 84: 8573-8577, 1987.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

25283 MW

Observed Molecular Weight

60 kDa

Applications Notes

6

Gene Name Synonym

Haptoglobin

Subcellular Location

Secreted.

Protein Name

Tumor necrosis factor receptor superfamily member 6

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.