Anti-GADD34/PPP1R15A Antibody Picoband®

About this product

Boster Bio Anti-GADD34/PPP1R15A Antibody Picoband® catalog # A02394-3. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02394-3

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Protein phosphatase 1 regulatory subunit 15A also known as growth arrest and DNA damage-inducible protein GADD34 is a protein that in humans is encoded by the PPP1R15A gene.

Synonyms

Caspase-2; CASP-2; Neural precursor cell expressed developmentally down-regulated protein 2; NEDD-2; Protease ICH-1; Caspase-2 subunit p18; Caspase-2 subunit p13; Caspase-2 subunit p12; CASP2; ICH1; NEDD2

Gene Name

PPP1R15A

Gene ID

23645

UniProt

O75807

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human GADD34/PPP1R15A, which shares 70.3% and 62.2% amino acid (aa) sequence identity with mouse and rat PPP1R15A, respectively.

Clonality

Polyclonal

Tissue Specificity

Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle.

Applications

WB

Field of Research

Apoptosis, Apoptotic Markers, Cancer, Caspases, Cell Biology, Cell Death, Intracellular, Invasion/Microenvironment, Metabolism, Metabolism Processes, Pathways and Processes, Proteolysis/Ubiquitin, Proteolytic Enzymes

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.

References & Citations

1. Blais, J. D., Filipenko, V., Bi, M., Harding, H. P., Ron, D., Koumenis, C., Wouters, B. G., Bell, J. C. Activating transcription factor 4 is translationally regulated by hypoxic stress. Molec. Cell. Biol. 24: 7469-7482, 2004. 2. Hasegawa, T., Yagi, A., Isobe, K. Interaction between GADD34 and kinesin superfamily, KIF3A. Biochem. Biophys. Res. Commun. 267: 593-596, 2000. 3. Hollander, M. C., Zhan, Q., Bae, I., Fornace, A. J., Jr. Mammalian GADD34, an apoptosis- and DNA damage-inducible gene. J. Biol. Chem. 272: 13731-13737, 1997.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

100 kDa

Gene Name Synonym

Caspase 2, apoptosis-related cysteine peptidase

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.