Anti-MCM7 Antibody Picoband® (monoclonal, 3H11)

About this product

Boster Bio Anti-MCM7 Antibody Picoband® (monoclonal, 3H11) catalog # M01649-3. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M01649-3

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

MCM7 (Minichromosome Maintenance, s. Cerevisiae, homolog of, 7), also called CDC47, FORMERLY, is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The MCM7 gene is mapped to 7q22.1. MCM7 plays a pivotal role in the G1/S phase transition, orchestrating the correct assembly of replication forks on chromosomal DNA and ensuring that all the genome is replicated once and not more than once at each cell cycle. The MCM7 gene contains 15 exons. The miRNAs MIR106B, MIR93, and MIR25 are clustered in a 5-prime to 3-prime orientation within intron 13. It has been found that MCM7 and the precursors of microRNAs (miRNAs) MIR106B, MIR93, and MIR25, all of which arise from intron 13 of the MCM7 gene, were overexpressed with almost perfect correlation in 5 of 10 human gastric tumors.

Synonyms

Alpha catenin antibody; alpha E catenin antibody; Alpha E-catenin antibody; alphaE catenin antibody; Cadherin associated protein 102kDa antibody; Cadherin associated protein antibody; Cadherin-associated protein antibody; CAP 102 antibody; CAP102 antibody; Catenin (cadherin associated protein) alpha 1 102kDa antibody; Catenin (cadherin associated protein), alpha 1, 102kDa antibody; Catenin alpha 1 antibody; Catenin alpha-1 antibody; CTNA1_HUMAN antibody; CTNNA 1 antibody; CTNNA1 antibody; FLJ36832 antibody; FLJ52416 antibody; NY REN 13 antigen antibody; OTTHUMP00000224141 antibody; OTTHUMP00000224147 antibody; Renal carcinoma antigen NY REN 13 antibody; Renal carcinoma antigen NY-REN-13 antibody

Gene Name

MCM7

Gene ID

4176

UniProt

P33993

Host

Mouse

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human MCM7 recombinant protein (Position: D526-V719) . Human MCM7 shares 94% amino acid (aa) sequence identity with MCM7.

Clonality

Monoclonal

Clone

Clone: 3H11

Tissue Specificity

Expressed ubiquitously in normal tissues.

Applications

WB, IHC, ICC, IF, Flow Cytometry

Field of Research

Actin Binding Proteins, Actin, etc., Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.

References & Citations

1. Groth, A., Corpet, A., Cook, A. J. L., Roche, D., Bartek, J., Lukas, J., Almouzni, G. Regulation of replication fork progression through histone supply and demand. Science 318: 1928-1931, 2007. 2. Nakatsuru, S., Sudo, K., Nakamura, Y. Isolation and mapping of a human gene (MCM2) encoding a product homologous to yeast proteins involved in DNA replication.Cytogenet. Cell Genet. 68: 226-230, 1995. 3. Petrocca, F., Visone, R., Onelli, M. R., Shah, M. H., Nicoloso, M. S., de Martino, I., Iliopoulos, D., Pilozzi, E., Liu, C.-G., Negrini, M., Cavazzini, L., Volinia, S., Alder, H., Ruco, L. P., Baldassarre, G., Croce, C. M., Vecchione, A. E2F1-regulated microRNAs impair TGF-beta-dependent cell-cycle arrest and apoptosis in gastric cancer. Cancer Cell 13: 272-286, 2008.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

81 kDa

Applications Notes

6

Gene Name Synonym

Catenin (cadherin-associated protein), alpha 1, 102kDa

Subcellular Location

Cytoskeleton. Cell membrane. Peripheral membrane protein. Cytoplasmic side. Adherens junction. Cell junction.

Protein Name

Activating transcription factor 1

Isotype

Mouse IgG1

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.