Anti-HLA-DR/HLA-DRA Antibody Picoband® (monoclonal, 8I10H1)

About this product

Boster Bio Anti-HLA-DR/HLA-DRA Antibody Picoband® (monoclonal, 8I10H1) catalog # M01195-3. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M01195-3

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

HLA class II histocompatibility antigen, DR alpha chainis aproteinthat in humans is encoded by the HLA-DRAgene. It is mapped to 6p21.32. HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages) . The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5.

Synonyms

Exportin-1; Exp1; Chromosome region maintenance 1 protein homolog; XPO1; CRM1

Gene Name

HLA-DRA

Gene ID

3122

UniProt

P01903

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human HLA-DR/HLA-DRA recombinant protein (Position: I26-L254) .

Clonality

Monoclonal

Clone

Clone: 8I10H1

Tissue Specificity

Expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. Not expressed in the kidney.

Applications

WB, IHC, Flow Cytometry

Field of Research

DNA/RNA, Epigenetics and Nuclear Signaling, Nuclear Import/Export, Nuclear Receptors, Nuclear Signaling Pathways, Protein Trafficking, RNA Processing, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP) . Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The ionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap. Several viruses, among them HIV-1, HTLV-1 and influenza A use it to export their unspliced or incompletely spliced RNAs out of the nucleus. Interacts with, and mediates the nuclear export of HIV-1 Rev and HTLV-1 Rex proteins. Involved in HTLV-1 Rex multimerization.

References & Citations

1. Tjernlund U, Scheynius A, Johansson C, et al. (1989) . T-cell response to purified protein derivative after removal of Langerhans' cells from epidermal cell suspensions containing keratinocytes expressing class II transplantation antigens. Scand. J. Immunol. 28 (6) : 667–73. 2. Chelladurai, M., Honn, K. V., Walz, D. A. HLA-DR is a procoagulant. Biochem. Biophys. Res. Commun. 178: 467-473, 1991. 3. Das, H. K., Lawrance, S. K., Weissman, S. M. Structure and nucleotide sequence of the heavy chain gene of HLA-DR. Proc. Nat. Acad. Sci. 80: 3543-3547, 1983.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

35-37 kDa

Applications Notes

6

Gene Name Synonym

Exportin 1

Subcellular Location

Cytoplasm. Nucleus, nucleoplasm. Nucleus, Cajal body. Nucleus, nucleolus. Located in the nucleoplasm, Cajal bodies and nucleoli. Shuttles between the nucleus/nucleolus and the cytoplasm.

Isotype

Mouse IgG1

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.