Anti-WDR1 Antibody Picoband® (monoclonal, 5C11C8)

About this product

Boster Bio Anti-WDR1 Antibody Picoband® (monoclonal, 5C11C8) catalog # M04814-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M04814-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

WD repeat-containing protein 1 is a protein that in humans is encoded by the WDR1 gene. It is mapped to 4p16.1. This gene encodes a protein containing 9 WD repeats. WD repeats are approximately 30- to 40-amino acid domains containing several conserved residues, mostly including a trp-asp at the C-terminal end. WD domains are involved in protein-protein interactions. The encoded protein may help induce the disassembly of actin filaments. Two transcript variants encoding different isoforms have been found for this gene.

Synonyms

Cofilin-2; Cofilin, muscle isoform; CFL2

Gene Name

WDR1

Gene ID

9948

UniProt

O75083

Host

Mouse

Reactivity

Human, Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human WDR1, different from the related mouse and rat sequences by one amino acid.

Clonality

Monoclonal

Clone

Clone: 5C11C8

Tissue Specificity

Isoform CFL2b is expressed predominantly in skeletal muscle and heart. Isoform CFL2a is expressed in various tissues.

Applications

IHC, WB

Field of Research

Cell Adhesion Proteins, Epigenetics and Nuclear Signaling, Integrins, Mediator Complex, Neuroscience, Receptors, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. Its F-actin depolymerization activity is regulated by association with CSPR3. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. Required for muscle maintenance. May play a role during the exchange of alpha-actin forms during the early postnatal remodeling of the sarcomere.

References & Citations

1. Entrez Gene: WDR1 WD repeat domain 1. 2. Adler HJ, Winnicki RS, Gong TW, Lomax MI (May 1999) . A gene upregulated in the acoustically damaged chick basilar papilla encodes a novel WD40 repeat protein. Genomics 56 (1) : 59–69. 3. Fujibuchi T, Abe Y, Takeuchi T, et al. (2005) . AIP1/WDR1 supports mitotic cell rounding. Biochem. Biophys. Res. Commun. 327 (1) : 268–75.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

66 kDa

Gene Name Synonym

Cofilin 2

Subcellular Location

Cytoskeleton. Nucleus matrix.

Isotype

Mouse IgG2b

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.