Anti-BRDT Antibody Picoband®

About this product

Boster Bio Anti-BRDT Antibody Picoband® catalog # A04985-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A04985-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Bromodomain testis-specific protein is a protein that in humans is encoded by the BRDT gene. BRDT is similar to the RING3 protein family. It possesses 2 bromodomain motifs and a PEST sequence (a cluster of proline, glutamic acid, serine, and threonine residues), characteristic of proteins that undergo rapid intracellular degradation. The bromodomain is found in proteins that regulate transcription. Several transcript variants encoding multiple isoforms have been found for this gene.

Synonyms

Transmembrane protein 107; TMEM107; DC20, UNQ638/PRO1268

Gene Name

BRDT

Gene ID

676

UniProt

Q58F21

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human BRDT recombinant protein (Position: Q795-D947) .

Clonality

Polyclonal

Tissue Specificity

Ubiquitous. .

Applications

ELISA, Flow Cytometry, WB

Field of Research

Cell Biology

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Plays a role in cilia formation and embryonic patterning. Requires for normal Sonic hedgehog (Shh) signaling in the neural tube and acts in combination with GLI2 and GLI3 to pattern ventral and intermediate neuronal cell types (By similarity) . .

References & Citations

1. Gaucher, J., Boussouar, F., Montellier, E., Curtet, S., Buchou, T., Bertrand, S., Hery, P., Jounier, S., Depaux, A., Vitte, A.-L., Guardiola, P., Pernet, K., Debernardi, A., Lopez, F., Holota, H., Imbert, J., Wolgemuth, D. J., Gerard, M., Rousseaux, S., Khochbin, S. Bromodomain-dependent stage-specific male genome programming by Brdt. EMBO J. 31: 3809-3820, 2012. 2. Jones, M. H., Numata, M., Shimane, M. Identification and characterization of BRDT: a testis-specific gene related to the bromodomain genes RING3 and Drosophila fsh. Genomics 45: 529-534, 1997. 3. Li, L., Sha, Y., Wang, X., Li, P., Wang, J., Kee, K., Wang, B. Whole-exome sequencing identified a homozygous BRDT mutation in a patient with acephalic spermatozoa. Oncotarget 8: 19914-19922, 2017.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

15503 MW

Observed Molecular Weight

180 kDa

Applications Notes

6

Gene Name Synonym

Transmembrane protein 107

Subcellular Location

Membrane ; Multi-pass membrane protein .

Protein Name

Transmembrane protein 107

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.