Anti-GLUD1/2 Antibody Picoband®

About this product

Boster Bio Anti-GLUD1/2 Antibody Picoband® catalog # A01866-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01866-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes glutamate dehydrogenase, which is a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid-induced insulin secretion. It is allosterically activated by ADP and inhibited by GTP and ATP. Activating mutations in this gene are a common cause of congenital hyperinsulinism. Alternative splicing of this gene results in multiple transcript variants. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Related pseudogenes have been identified on chromosomes 10, 18 and X.

Synonyms

UDP-glucuronosyltransferase 1-1; UDPGT 1-1; UGT1*1; UGT1-01; UGT1.1; Bilirubin-specific UDPGT isozyme 1; hUG-BR1; UDP-glucuronosyltransferase 1-A; UGT-1A; UGT1A; UDP-glucuronosyltransferase 1A1; UGT1A1; GNT1; UGT1

Gene Name

GLUD1/2

Gene ID

2746/2747

UniProt

P00367/P49448

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human GLUD1/2 recombinant protein (Position: S54-A553) .

Clonality

Polyclonal

Tissue Specificity

Isoform 1 and isoform 2 are expressed in liver, colon and small intestine. Isoform 2 but not isoform 1 is expressed in kidney. Isoform 1 and isoform 2 are not expressed in esophagus. Not expressed in skin.

Applications

WB, IHC, ICC, IF, Flow Cytometry, ELISA

Field of Research

Drug Metabolism, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isoform glucuronidates bilirubin IX- alpha to form both the IX-alpha-C8 and IX-alpha-C12 monoconjugates and diconjugate. Is also able to catalyze the glucuronidation of 17beta-estradiol, 17alpha-ethinylestradiol, 1-hydroxypyrene, 4- methylumbelliferone, 1-naphthol, paranitrophenol, scopoletin, and umbelliferone. Isoform 2 lacks transferase activity but acts as a negative regulator of isoform 1.

References & Citations

1. Anagnou, N. P., Seuanez, H., Modi, W., O'Brien, S. J., Papmatheakis, J., Moschonas, N. Chromosomal mapping of the human glutamate dehydrogenase (GLUD) genes to chromosomes 10q21.1-21.2 and Xq26-28. (Abstract) Am. J. Hum. Genet. 45 (suppl.) : A170 only, 1989. 2. Barbeau, A., Charbonneau, M., Cloutier, T. Leucocyte glutamate dehydrogenase in various hereditary ataxias. Canad. J. Neurol. Sci. 7: 421-424, 1980. 3. Colon, A. D., Plaitakis, A., Perakis, A., Berl, S., Clarke, D. D. Purification and characterization of a soluble and a particulate glutamate dehydrogenase from rat brain. J. Neurochem. 46: 1811-1819, 1986.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

50433 MW

Observed Molecular Weight

54 kDa

Applications Notes

6

Gene Name Synonym

UDP glucuronosyltransferase family 1 member A1

Subcellular Location

Isoform 1: Microsome.

Protein Name

Tubulin beta chain

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.