Anti-NDUFS2 Antibody Picoband®

About this product

Boster Bio Anti-NDUFS2 Antibody Picoband® catalog # A05618-3. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A05618-3

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial (NDUFS2) also known as NADH-ubiquinone oxidoreductase 49 kDa subunit is an enzyme that in humans is encoded by the NDUFS2 gene. The protein encoded by this gene is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I) . Mammalian mitochondrial complex I is composed of at least 43 different subunits, 7 of which are encoded by the mitochondrial genome, and the rest are the products of nuclear genes. The iron-sulfur protein fraction of complex I is made up of 7 subunits, including this gene product. Complex I catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria. Mutations in this gene are associated with mitochondrial complex I deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Synonyms

Transcription factor jun-D; JUND

Gene Name

NDUFS2

Gene ID

4720

UniProt

O75306

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human NDUFS2 recombinant protein (Position: M1-R463) .

Clonality

Polyclonal

Applications

WB, IHC, ICC, IF, Flow Cytometry, ELISA

Field of Research

Cancer, Cancer Susceptibility, Cardiovascular, Domain Families, Epigenetics and Nuclear Signaling, Hlh/Leucine Zipper, Hypertrophy, Immunology, Innate Immunity, Oncoproteins, Oncoproteins/Suppressors, Proto-Oncogenes, TLR Signaling, Transcription, Transcription Factors

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Transcription factor binding AP-1 sites.

References & Citations

1. Carilla-Latorre, S., Gallardo, M. E., Annesley, S. J., Calvo-Garrido, J., Grana, O., Accari, S. L., Smith, P. K., Valencia, A., Garesse, R., Fisher, P. R., Escalante, R. MidA is a putative methyltransferase that is required for mitochondrial complex I function. J. Cell Sci. 123: 1674-1683, 2010. 2. Loeffen, J., Elpeleg, O., Smeitink, J., Smeets, R., Stockler-Ipsiroglu, S., Mandel, H., Sengers, R., Trijbels, F., van den Heuvel, L. Mutations in the complex I NDUFS2 gene of patients with cardiomyopathy and encephalomyopathy. Ann. Neurol. 49: 195-201, 2001. 3. Loeffen, J., van den Heuvel, L., Smeets, R., Triepels, R., Sengers, R., Trijbels, F., Smeitink, J. cDNA sequence and chromosomal localization of the remaining three human nuclear encoded iron sulphur protein (IP) subunits of complex I: the human IP fraction is completed. Biochem. Biophys. Res. Commun. 247: 751-758, 1998.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

45 kDa

Gene Name Synonym

JunD proto-oncogene, AP-1 transcription factor subunit

Subcellular Location

Nucleus.

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.