Anti-NOP58 Antibody Picoband®

About this product

Boster Bio Anti-NOP58 Antibody Picoband® catalog # A05070-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A05070-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Nucleolar protein 58 is a protein that in humans is encoded by the NOP58 gene. The protein encoded by this gene is a core component of box C/D small nucleolar ribonucleoproteins. Some box C/D small nucleolar RNAs (snoRNAs), such as U3, U8, and U14, are dependent upon the encoded protein for their synthesis. This protein is SUMOylated, which is necessary for high affinity binding to snoRNAs.

Synonyms

Matrix metalloproteinase-16; MMP-16; 3.4.24.-; MMP-X2; Membrane-type matrix metalloproteinase 3; MT-MMP 3; MTMMP3; Membrane-type-3 matrix metalloproteinase; MT3-MMP; MT3MMP; MMP16; MMPX2

Gene Name

NOP58

Gene ID

51602

UniProt

Q9Y2X3

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human NOP58 recombinant protein (Position: K30-K450) .

Clonality

Polyclonal

Tissue Specificity

Expressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary.

Applications

WB, ICC, IF, Flow Cytometry, ELISA

Field of Research

Angiogenesis, Cancer, Cardiovascular, Cell Biology, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Metalloprotease, Proteolysis/Ubiquitin, Proteolytic Enzymes

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells.

References & Citations

1. Hartz, P. A. Personal Communication. Baltimore, Md. 1/8/2016. 2. Lyman, S. K., Gerace, L., Baserga, S. J. Human Nop5/Nop58 is a component common to the box C/D small nucleolar ribonucleoproteins. RNA 5: 1597-1604, 1999. 3. Nelson, S. A., Santora, K. E., LaRochelle, W. J. Isolation and characterization of a novel PDGF-induced human gene. Gene 253: 87-93, 2000.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

39125 MW

Observed Molecular Weight

70 kDa

Gene Name Synonym

Matrix metallopeptidase 16

Subcellular Location

Isoform Long: Cell membrane ; Localized at the cell surface of melanoma cells.

Protein Name

RNA-binding protein Musashi homolog 1

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.