Anti-BAG2 Antibody Picoband® (monoclonal, 8F11G2)

About this product

Boster Bio Anti-BAG2 Antibody Picoband® (monoclonal, 8F11G2) catalog # M04933-2. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M04933-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

BAG family molecular chaperone regulator 2 is a protein that in humans is encoded by the BAG2 gene. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. The functional antagonisms displayed between BAG family proteins and Hip suggest that a proper balance of these 2 types of protein is required for achieving optimal cycles of substrate binding and release required for inducting conformational changes in proteins, with Hip promoting peptide substrate binding by Hsc70/Hsp70 and BAG family proteins promoting dissociation.

Synonyms

Cofilin-2; Cofilin, muscle isoform; CFL2

Gene Name

BAG2

Gene ID

9532

UniProt

O95816

Host

Mouse

Reactivity

Human, Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human BAG2 recombinant protein (Position: M1-N211) . Human BAG2 shares 93.4% amino acid (aa) sequence identity with mouse BAG2.

Clonality

Monoclonal

Clone

Clone: 8F11G2

Tissue Specificity

Isoform CFL2b is expressed predominantly in skeletal muscle and heart. Isoform CFL2a is expressed in various tissues.

Applications

WB, Flow Cytometry

Field of Research

Cell Adhesion Proteins, Epigenetics and Nuclear Signaling, Integrins, Mediator Complex, Neuroscience, Receptors, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. Its F-actin depolymerization activity is regulated by association with CSPR3. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. Required for muscle maintenance. May play a role during the exchange of alpha-actin forms during the early postnatal remodeling of the sarcomere.

References & Citations

1. Takayama S, Xie Z, Reed JC (Feb 1999) . An evolutionarily conserved family of Hsp70/Hsc70 molecular chaperone regulators. J Biol Chem 274 (2) : 781–6. 2. Entrez Gene: BAG2 BCL2-associated athanogene 2.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

24 kDa

Applications Notes

6

Gene Name Synonym

Cofilin 2

Subcellular Location

Cytoskeleton. Nucleus matrix.

Isotype

Mouse IgG1

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.