Anti-gamma Catenin Antibody Picoband® (monoclonal, 4C12D7)

About this product

Boster Bio Anti-gamma Catenin Antibody Picoband® (monoclonal, 4C12D7) catalog # M01901-3. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M01901-3

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Junction plakoglobin (JUP), also known as gamma-catenin, is a protein that in humans is encoded by the JUP gene. It is a member of the catenin protein family and homologous to β-catenin, and it is mapped to 17q21.2. This gene encodes a major cytoplasmic protein that is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. This protein forms distinct complexes with cadherins and desmosomal cadherins. Meanwhile, JUP may have distinct roles in Wnt signaling and cancer via differential effects on downstream target genes.

Synonyms

Ras-related protein Rab-5A; RAB5A; RAB5

Gene Name

JUP

Gene ID

3728

UniProt

P14923

Host

Mouse

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human gamma Catenin recombinant protein (Position: M556-A745) . Human gamma Catenin shares 98% amino acid (aa) sequence identity with both mouse and rat gamma Catenin.

Clonality

Monoclonal

Clone

Clone: 4C12D7

Tissue Specificity

Widely expressed in fetal and adult tissues.

Applications

WB, IHC, ICC, IF, Flow Cytometry

Field of Research

Cell Type Marker, Neuron Marker, Neuroscience, Neurotransmission, Organelles, Protein Trafficking, Secretory Vesicles, Signal Transduction, Subcellular Markers, Synapse Marker, Tags & Cell Markers, Vesicle Transport

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors ly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan. Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3.

References & Citations

1. Kolligs, F. T.; Kolligs, B.; Hajra, K. M.; Hu, G.; Tani, M.; Cho, K. R.; Fearon, E. R. : Gamma-catenin is regulated by the APC tumor suppressor and its oncogenic activity is distinct from that of beta-catenin. Genes Dev. 14: 1319-1331, 2000. 2. Entrez Gene: JUP junction plakoglobin

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

25035 MW

Observed Molecular Weight

82 kDa

Applications Notes

6

Gene Name Synonym

RAB5A, member RAS oncogene family

Subcellular Location

Early endosome membrane. Lipid-anchor. Endosome membrane. Cytosol. Cell membrane. Cytoplasmic side. Melanosome. Cytoplasmic vesicle. Ruffle. Membrane. Phagosome membrane.

Isotype

Mouse IgG1

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.