Anti-HSF1 Antibody Picoband®

About this product

Boster Bio Anti-HSF1 Antibody Picoband® catalog # A00250. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00250

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

HSF1 (HEAT-SHOCK TRANSCRIPTION FACTOR 1), also called HEAT-SHOCK FACTOR 1, is a protein that in humans is encoded by the HSF1 gene. The product of HSF1 gene is a heat-shock transcription factor. The International Radiation Hybrid Mapping Consortium maps the HSF1 gene to chromosome 8. HSF1 exists as an inactive monomer in a complex with Hsp40/Hsp70 and Hsp90. Upon stress, such as elevated temperature, HSF1 is released from the chaperone complex and trimerizes. HSF1 is then transported into the nucleus where it is hyperphosphorylated and binds to DNA containing heat shock elements. HSF1's target genes include major inducible heat shock proteins such as Hsp72, and interestingly, noncoding RNA within Satellite III repeat regions. In a novel in vitro system human HSF1 can be activated by nonnative protein, heat, and geldanamycin. HSR1 is constitutively expressed in human and rodent cells and its homologs are functionally interchangeable. Furthermore, Hsf1-deficient mice had a longer free-running period than wildtype littermates.

Synonyms

C-reactive protein; C-reactive protein (1-205) ; CRP; PTX1

Gene Name

HSF1

Gene ID

3297

UniProt

Q00613

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human HSF1, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Tissue Specificity

Found in plasma.

Applications

WB, IHC, Flow Cytometry

Field of Research

Apoptosis, Atherosclerosis, Cardiovascular, Chromatin Binding Proteins, Complement, DNA/RNA Binding, Epigenetics and Nuclear Signaling, Immunology, Innate Immunity, Macrophage/Inflammation, Metabolism, Vascular Inflammation

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

References & Citations

1. Christians, E., Davis, A. A., Thomas, S. D., Benjamin, I. J. Maternal effect of Hsf1 on reproductive success. Nature 407: 693-694, 2000. 2. Schuetz, T. J. Personal Communication. Boston, Mass. 10/3/1991.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

80 kDa

Gene Name Synonym

C-reactive protein

Subcellular Location

Secreted.

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.