Anti-Pannexin 1/PANX1 Antibody Picoband®

About this product

Boster Bio Anti-Pannexin 1/PANX1 Antibody Picoband® catalog # A00915-2. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00915-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Pannexin 1 is a protein in humans that is encoded by the PANX1 gene. The protein encoded by this gene belongs to the innexin family. Innexin family members are the structural components of gap junctions. This protein and pannexin 2 are abundantly expressed in central nerve system (CNS) and are coexpressed in various neuronal populations. Studies in Xenopus oocytes suggest that this protein alone and in combination with pannexin 2 may form cell type-specific gap junctions with distinct properties.

Synonyms

CD59A glycoprotein; MAC-inhibitory protein; MAC-IP; Membrane attack complex inhibition factor; MACIF; Protectin; CD59; Cd59a; Cd59

Gene Name

PANX1

Gene ID

24145

UniProt

Q96RD7

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Pannexin 1/PANX1 recombinant protein (Position: M1-D362) .

Clonality

Polyclonal

Tissue Specificity

Detected in T-47D, MDA-MB-175 and HBL-100 breast carcinoma cells, A-431 epidermoid carcinoma cells, SW48 and SNU-C2B colon carcinoma cells and Hs 294T melanoma cells (at protein level) . Expressed at low levels in most adult tissues and is highest in the brain, lung, placenta and kidney. Lower levels of expression are detected in melanocytes, heart, liver, skeletal muscle and pancreas. Abundant in breast carcinoma cell lines. In the colonic mucosa, expressed in epithelia but not in the connective tissue of the lamina propria. In the thyroid gland, expressed in the epithelium of the thyroid follicles. In pancreas, expressed in the islets of Langerhans cells, but not in the surrounding epithelial cells of the exocrine pancreas. In kidney, expressed in the epithelia of the distal tubules. Not expressed in connective tissue, endothelial cells, adipose tissue, muscle cells or cells of hematopoietic origin.

Applications

WB, ICC, IF, Flow Cytometry, ELISA

Field of Research

Angiogenesis, Cancer, Cancer Metabolism, Cardiovascular, Growth Factors, Growth Factors/Hormones, Hematopoietic Progenitors, Host-Virus Interaction, Interspecies Interaction, Invasion/Microenvironment, Metabolism, Metabolism Processes, Microbiology, Oncoproteins, Oncoproteins/Suppressors, Pathways and Processes, Protein Phosphorylation, Receptor Tyrosine Kinases, Response To Hypoxia, Signal Transduction, Stem Cells, Surface Molecules, Tyrosine Kinases

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore (By similarity) .

References & Citations

1. Bruzzone, R., Hormuzdi, S. G., Barbe, M. T., Herb, A., Monyer, H. Pannexins, a family of gap junction proteins expressed in brain. Proc. Nat. Acad. Sci. 100: 13644-13649, 2003. 2. Chekeni, F. B., Elliott, M. R., Sandilos, J. K., Walk, S. F., Kinchen, J. M., Lazarowski, E. R., Armstrong, A. J., Penuela, S., Laird, D. W., Salvesen, G. S., Isakson, B. E., Bayliss, D. A., Ravichandran, K. S. Pannexin 1 channels mediate 'find-me' signal release and membrane permeability during apoptosis. Nature 467: 863-867, 2010. 3. Gross, M. B. Personal Communication. Baltimore, Md. 11/30/2016.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

48 kDa

Applications Notes

6

Gene Name Synonym

CD59a antigen

Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor.

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.